Lehto 2022 Neurochem Res: Difference between revisions
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|title=Lehto A, Koch K, Barnstorf-Brandes J, Viel C, Fuchs M, Klein J (2022) ß-Hydroxybutyrate improves mitochondrial function after transient ischemia in the mouse. https://doi.org/10.1007/s11064-022-03637-6 | |title=Lehto A, Koch K, Barnstorf-Brandes J, Viel C, Fuchs M, Klein J (2022) ß-Hydroxybutyrate improves mitochondrial function after transient ischemia in the mouse. https://doi.org/10.1007/s11064-022-03637-6 | ||
|info=Neurochem Res 47:3241-49. [https://pubmed.ncbi.nlm.nih.gov/35674929 PMID: 35674929 Open Access] | |info=Neurochem Res 47:3241-49. [https://pubmed.ncbi.nlm.nih.gov/35674929 PMID: 35674929 Open Access] | ||
|authors=Lehto | |authors=Lehto Allna, Koch Konrad, Barnstorf-Brandes Johanna, Viel Christian, Fuchs Marius, Klein Jochen | ||
|year=2022 | |year=2022 | ||
|journal=Neurochem Res | |journal=Neurochem Res | ||
|abstract=ß-Hydroxybutyrate (BHB) is a ketone body formed in high amounts during lipolysis and fasting. Ketone bodies and the ketogenic diet were suggested as neuroprotective agents in neurodegenerative disease. In the present work, we induced transient ischemia in mouse brain by unilaterally occluding the middle cerebral artery for 90 min. BHB (30 mg/kg), given immediately after reperfusion, significantly improved the neurological score determined after 24 h. In isolated mitochondria from mouse brain, oxygen consumption by the complexes I, II and IV was reduced immediately after ischemia but recovered slowly over 1 week. The single acute BHB administration after reperfusion improved complex I and II activity after 24 h while no significant effects were seen at later time points. After 24 h, plasma and brain BHB concentrations were strongly increased while mitochondrial intermediates (citrate, succinate) were unchanged in brain tissue. Our data suggest that a single administration of BHB may improve mitochondrial respiration for 1-2 days but not for later time points. Endogenous BHB formation seems to complement the effects of exogenous BHB administration. | |abstract=ß-Hydroxybutyrate (BHB) is a ketone body formed in high amounts during lipolysis and fasting. Ketone bodies and the ketogenic diet were suggested as neuroprotective agents in neurodegenerative disease. In the present work, we induced transient ischemia in mouse brain by unilaterally occluding the middle cerebral artery for 90 min. BHB (30 mg/kg), given immediately after reperfusion, significantly improved the neurological score determined after 24 h. In isolated mitochondria from mouse brain, oxygen consumption by the complexes I, II and IV was reduced immediately after ischemia but recovered slowly over 1 week. The single acute BHB administration after reperfusion improved complex I and II activity after 24 h while no significant effects were seen at later time points. After 24 h, plasma and brain BHB concentrations were strongly increased while mitochondrial intermediates (citrate, succinate) were unchanged in brain tissue. Our data suggest that a single administration of BHB may improve mitochondrial respiration for 1-2 days but not for later time points. Endogenous BHB formation seems to complement the effects of exogenous BHB administration. | ||
|keywords=Complex I, Complex II, Glucose, Microdialysis, Oxidative phosphorylation, Stroke | |||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Pharmacology;toxicology | ||
|diseases=Neurodegenerative | |||
|organism=Mouse | |||
|tissues=Nervous system | |||
|preparations=Isolated mitochondria | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2023-05 | |additional=2023-05 | ||
}} | }} | ||
Revision as of 14:02, 2 May 2023
| Lehto A, Koch K, Barnstorf-Brandes J, Viel C, Fuchs M, Klein J (2022) ß-Hydroxybutyrate improves mitochondrial function after transient ischemia in the mouse. https://doi.org/10.1007/s11064-022-03637-6 |
» Neurochem Res 47:3241-49. PMID: 35674929 Open Access
Lehto Allna, Koch Konrad, Barnstorf-Brandes Johanna, Viel Christian, Fuchs Marius, Klein Jochen (2022) Neurochem Res
Abstract: ß-Hydroxybutyrate (BHB) is a ketone body formed in high amounts during lipolysis and fasting. Ketone bodies and the ketogenic diet were suggested as neuroprotective agents in neurodegenerative disease. In the present work, we induced transient ischemia in mouse brain by unilaterally occluding the middle cerebral artery for 90 min. BHB (30 mg/kg), given immediately after reperfusion, significantly improved the neurological score determined after 24 h. In isolated mitochondria from mouse brain, oxygen consumption by the complexes I, II and IV was reduced immediately after ischemia but recovered slowly over 1 week. The single acute BHB administration after reperfusion improved complex I and II activity after 24 h while no significant effects were seen at later time points. After 24 h, plasma and brain BHB concentrations were strongly increased while mitochondrial intermediates (citrate, succinate) were unchanged in brain tissue. Our data suggest that a single administration of BHB may improve mitochondrial respiration for 1-2 days but not for later time points. Endogenous BHB formation seems to complement the effects of exogenous BHB administration. • Keywords: Complex I, Complex II, Glucose, Microdialysis, Oxidative phosphorylation, Stroke • Bioblast editor: Plangger M
Labels: MiParea: Respiration, Pharmacology;toxicology
Pathology: Neurodegenerative
Organism: Mouse Tissue;cell: Nervous system Preparation: Isolated mitochondria
HRR: Oxygraph-2k
2023-05
