From Bioblast
Locating a problem |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Switch components
Problem moves with | Problem situated at |
---|---|
POS | POS |
POS connector | POS connector |
not at all | Chamber assembly or Main unit |
Remove components
State | Description | Expected behavior |
I | fully assembled, sensor in chamber, air saturation, Gain =2 | stable signal, 3 to 5 V |
II | POS connector + sensor removed from chamber, sensor still connected to sensor connector | initial change in voltage, new, stable non-zero level of signal reached |
III | sensor removed from POS connector | stable signal at 0 V |
IV | POS connector removed from main unit | for oxygraphs series A to C: typically signal off scale (+/- 9.99 V), for oxygraphs series D and higher: typically 0 V; may vary, a gain independent stable signal of 0.4 V is a strong indication of an electronic problem in the main unit |
State expected behavior observed? yes/no |
Interpretation / Comment | |||
---|---|---|---|---|
I | II | III | IV | |
no | yes | yes | yes | may indicate a problem in the Chamber assembly, or a defective sensor, continue testing |
no | no/yes | yes | yes | probable defect in the Sensor. This indication is not so strong as a localization obtained via switching components. The POS connector (not the Sensor) may be defect, but this may be only visible when a signal is present. |
no | no | no | yes | indicates a defect in the POS connector |
no | no | no | no | indicates a problem with Chamber assembly or a defect in the Main unit; if the problem can not be solved by reassembling the chamber, contact OROBOROS Instruments. |