Locating a problem

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O2k-Open Support

Locating a problem



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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Switch components

Summary
Problem moves with Problem situated at
POS POS
POS connector POS connector
not at all Chamber assembly or Main unit


Remove components

Tested states and expected behavior
State Description Expected behavior
I fully assembled, sensor in chamber, air saturation, Gain =2 stable signal, 3 to 5 V
II POS connector + sensor removed from chamber, sensor still connected to sensor connector initial change in voltage, new, stable non-zero level of signal reached
III sensor removed from POS connector stable signal at 0 V
IV POS connector removed from main unit for oxygraphs series A to C: typically signal off scale (+/- 9.99 V), for oxygraphs series D and higher: typically 0 V; may vary, a gain independent stable signal of 0.4 V is a strong indication of an electronic problem in the main unit


State
expected behavior observed? yes/no
Interpretation / Comment

I II III IV
no yes yes yes may indicate a problem in the Chamber assembly, or a defective sensor, continue testing
no no/yes yes yes probable defect in the Sensor. This indication is not so strong as a localization obtained via switching components. The POS connector (not the Sensor) may be defect, but this may be only visible when a signal is present.
no no no yes indicates a defect in the POS connector
no no no no indicates a problem with Chamber assembly or a defect in the Main unit; if the problem can not be solved by reassembling the chamber, contact OROBOROS Instruments.


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