Malate-anaplerotic pathway control state: Difference between revisions
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Revision as of 15:33, 25 January 2016
- high-resolution terminology - matching measurements at high-resolution
Malate-anaplerotic pathway control state
Description
M: Malate alone does not support respiration of mt-preparations if oxaloacetate cannot be metabolized further in the absence of a source of acetyl-CoA. Transport of oxaloacetate across the inner mt-membrane is restricted particularly in liver. Mitochondrial citrate and 2-oxoglutarate (α-ketoglutarate) are depleted by antiport with malate. Succinate is lost from the mitochondria through the dicarboxylate carrier.
Abbreviation: M
Reference: Gnaiger 2014 MitoPathways - Chapter 3.1
M(L)
M(P)
M(E)
Details
OXPHOS capacity with malate alone is only 1.3% of that with Pyruvate&Malate in isolated rat skeletal muscle mitochondria. Many mammalian and non-mammalian mitochondria have a mt-isoform of NADP+- or NAD(P)+-dependent malic enzyme (ME), the latter being particularly active in proliferating cells. Then malate alone can support high respiratory activities.
