MiPNet06.03 POS-calibration-SOP: Difference between revisions

Latest revision as of 14:27, 27 July 2023

MiPNet06.03 POS-calibration-SOP

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.

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Oroboros (2023-02-06) Mitochondr Physiol Network

Abstract: Gnaiger E (2023) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(21):1-10.

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

Further details: » MitoPedia: O2k-Open Support

• O2k-Network Lab: AT_Innsbruck_Oroboros

Keywords

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

O2k-Procedures

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen calibration - DatLab

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

» Oxygen calibration - DatLab

Labels: MiParea: Respiration, Instruments;methods

HRR: Oxygraph-2k, O2k-Manual

DatLab, O2k-SOP, DL7, Oxygen solubility, DL8, MitoFit 2021 Dark respiration, MitoFit 2021 Photosynthesis, DL8, PLoSONE2022ace-sce, MitoFit2022QC, POS

@@ Line 1: / Line 1: @@
+{{Template:OROBOROS support page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://www.oroboros.at/?O2k-Protocols|O2k-Protocols contents]]Fasching M, Gnaiger E (2014) Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Mitochondr Physiol Network 06.03(12): 1-8.
+|title=[[Image:O2k-Manual.jpg|right|70px|link=O2k-Manual|O2k-Manual]]O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.
-|info=[[Media:MiPNet06.03 POS-Calibration-SOP.pdf|'''Open Access-MiPNet06.03.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions]
+|info=[[File:PDF.jpg|100px|link=https://wiki.oroboros.at/images/7/77/MiPNet06.03_POS-Calibration-SOP.pdf |Bioblast pdf]]  » [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions]
-|authors=OROBOROS
+|authors=Oroboros
-|year=2014-03
+|year=2023-02-06
 |journal=Mitochondr Physiol Network
-|abstract=[[High-resolution respirometry]] critically depends on accurate calibration of the [[polarographic oxygen sensor]]s which respond to [[Oxygen_pressure| partial oxygen pressure]].  Expressing the oxygen signal in terms of partial pressure has several advantages, but respiration is expressed in molar units related to biochemical stoichiometries. Conversion of oxygen partial pressure to [[oxygen concentration]] requires accurate information on oxygen solubilities in experimental incubation media. Absolute errors up to 10% are commonly found in the established literature. The [[oxygen solubility]] of mitochondrial respiration medium [[MiR06]] relative to pure water (oxygen solubility factor, ''F''<sub>M</sub>) is 0.92, accurately determined (for MiR05, i.e. MiR06 without catalase; [[MiPNet14.13]]) at 37 °C and 30 °C.  At air saturation at standard barometric pressure (100 kPa) and 37 °C, the partial oxygen pressure is 19.63 kPa, and the oxygen concentration is 190.7 µM in MiR06.  A clean and ready-to-use Oxygraph chamber is a requirement for saving time and quickly calibrating the polarographic oxygen sensors.
+|abstract=Gnaiger E (2023) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(21):1-10.
+{{MiPNet pdf page linking to MitoPedia}}
-:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]
+|mipnetlab=AT_Innsbruck_Oroboros
-:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
-|mipnetlab=AT_Innsbruck_OROBOROS
-}}
-{{Labeling
-|area=Instruments;methods
-|instruments=Oxygraph-2k, Protocol
-|additional=O2k-SOP
 }}
-== Further details ==
-:>> [[MiPNet12.08 O2k-Calibration]]
-:>> [[MiPNet14.06 InstrumentalBackground]]
-:>> [http://www.bioblast.at/images/8/82/Forstner_1983_POS.pdf Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York: 321-333.]
+== Keywords ==
+{{Template:Keywords: Oxygen signal}}
-[[Image:MiPNet06.03_Table.jpg|centre|frame|<sup>c</sup>O2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. FM has been determined for MiR05 at 30 °C and 37 °C, and is assumed to be identical at 25 °C.]]
+::::» [[Oxygen calibration - DatLab]]
-=== This article is cited in ===
+{{Labeling
+|area=Respiration, Instruments;methods
-* [http://www.ncbi.nlm.nih.gov/pubmed/18370948 Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-328]
+|instruments=Oxygraph-2k, O2k-Manual
+|additional=DatLab, O2k-SOP, DL7, Oxygen solubility, DL8,
+MitoFit 2021 Dark respiration, MitoFit 2021 Photosynthesis, DL8, PLoSONE2022ace-sce, MitoFit2022QC, POS
+}}