MiPNet17.17 Amplex-Mouse-brain: Difference between revisions

Revision as of 16:44, 10 January 2013

Has title::Fasching M (2012) O2k-Fluorometry - A MitoCom Project. Mitochondr Physiol Network 17.17.

» [[Has info::MiPNet17.17]]

Was written by::Fasching M, Was written by::Sumbalova Z, Was written by::Gnaiger E (Was published in year::2012) Was published in journal::Mitochondr Physiol Network

Abstract: [[has abstract::High-resolution respirometry [1] is extended by MultiSensor techniques allowing the simultaneous measurement of oxygen consumption and one additional parameter (mitochondrial membrane potential, pH, Ca2+, or NO concentration [2, 3]). Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H2O2 production, mitochondrial membrane potential, intracellular pH, Ca2+, Mg2+ , and NADH levels. Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:

Quality control of preparations by respirometric performance.
Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP+ or fluorophores.
Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
Additional information is acquired for a limited amount of sample.
Oxygen dependence of parameters can be studied.

Here we introduce the O2k-Fluorescence LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H2O2 production of mouse brain mitochondria.]] • Keywords: has publicationkeywords::Oxygraph-2k, has publicationkeywords::High-resolution respirometry, has publicationkeywords::Spectrofluorometry, has publicationkeywords::MitoCom

• O2k-Network Lab: Was published by MiPNetLab::AT_Innsbruck_OROBOROS

Labels:

HRR: Instrument and method::Oxygraph-2k, Instrument and method::Spectrofluorimetry, Instrument and method::MiPNet-Publication

@@ Line 1: / Line 1: @@
 {{Publication
-|title=Harrison DK (2012) O2k-Spectrophotometry - A MitoCom Project. Mitochondr Physiol Network 17.16.
+|title=Fasching M (2012) O2k-Fluorometry - A MitoCom Project. Mitochondr Physiol Network 17.17.
-|info=[http://www.oroboros.at/index.php?id=o2k-spectrophotometry MiPNet17.16]
+|info=[http://www.oroboros.at/index.php?id=o2k-fluorescenceled2-module MiPNet17.17]
-|authors=Harrison DK, Fasching M, Gnaiger E
+|authors=Fasching M, Sumbalova Z, Gnaiger E
 |year=2012
 |journal=Mitochondr Physiol Network
-|abstract=The principal thrust of the technical development of the O2k high-resolution respirometer within the MitoCom project is towards the O2k-Fluorometer. However, simultaneously, a parallel project is working towards the integration of spectrophotometry into the O2k in order to measure the redox state of cytochromes – particularly cytochromes c and aa3, but also cytochrome b.
+|abstract=High-resolution respirometry [1] is extended by MultiSensor techniques allowing the simultaneous measurement of oxygen consumption and one additional parameter (mitochondrial membrane potential, pH, Ca2+, or NO concentration [2, 3]). Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H2O2 production, mitochondrial membrane potential, intracellular pH, Ca2+, Mg2+ , and NADH levels.
-|keywords=Oxygraph-2k, High-resolution respirometry, Spectrophotometry, MitoCom
+Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
+# Quality control of preparations by respirometric performance.
+# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP+ or fluorophores.
+# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
+# Additional information is acquired for a limited amount of sample.
+# Oxygen dependence of parameters can be studied.
+Here we introduce the O2k-Fluorescence LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H2O2 production of mouse brain mitochondria.
+|keywords=Oxygraph-2k, High-resolution respirometry, Spectrofluorometry, MitoCom
 |mipnetlab=AT_Innsbruck_OROBOROS
 |articletype=Workshop, MiPNet-online Publication
 }}
 {{Labeling
-|instruments=Oxygraph-2k, Spectrophotometry, MiPNet-Publication
+|instruments=Oxygraph-2k, Spectrofluorimetry, MiPNet-Publication
 |articletype=Workshop, MiPNet-online Publication
 }}