MiPNet18.06 Amplex-Yeast: Difference between revisions

Revision as of 16:05, 25 November 2018

MiPNet18.06 Amplex-Yeast

O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast.

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Oroboros (2018-11-25) Mitochondr Physiol Network

Abstract: Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2018) O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(05):1-4.

>> Product: Oroboros O2k, O2k-Catalogue

• O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom

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 {{OROBOROS header page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast.
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast.
 |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/f/f9/MiPNet18.06_Amplex-Yeast.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet18.06_Amplex-Yeast.pdf Versions]
 |authors=Oroboros
-|year=2015-06-15
+|year=2018-11-25
 |journal=Mitochondr Physiol Network
-|abstract='''Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2015) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(04):1-4'''
+|abstract='''Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2018) O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(05):1-4.'''
-Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the [[O2k-Fluorescence LED2-Module]]. Extracellular H<sub>2</sub>O<sub>2</sub>-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H<sub>2</sub>O<sub>2</sub> production (Fig. 2).
 :>> Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]]
 |mipnetlab=AT Innsbruck Oroboros, AT Innsbruck MitoCom
 }}
+== See also ==
+:::* A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. - [[MiPNet15.09. Yeast: HRR Reference Assay |»Bioblast link«]]
 {{Labeling
 |area=Respiration
@@ Line 21: / Line 22: @@
 |additional=O2k-Demo, O2k-MultiSensor
 }}
-== See also ==
-* A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. - [[MiPNet15.09. Yeast: HRR Reference Assay |»Bioblast link«]]