Difference between revisions of "MiPNet18.06 Amplex-Yeast"
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{{Publication | {{Publication | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. | |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. | ||
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/f/f9/MiPNet18.06_Amplex-Yeast.pdf |Bioblast pdf]] | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/f/f9/MiPNet18.06_Amplex-Yeast.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet18.06_Amplex-Yeast.pdf Versions] | ||
|authors=Oroboros | |authors=Oroboros | ||
|year=2015-06-15 | |year=2015-06-15 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2015) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(04):1-4''' | |abstract='''Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2015) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(04):1-4''' | ||
Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the [[O2k-Fluorescence LED2-Module]]. Extracellular H<sub>2</sub>O<sub>2</sub>-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H<sub>2</sub>O<sub>2</sub> production (Fig. 2). | Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the [[O2k-Fluorescence LED2-Module]]. Extracellular H<sub>2</sub>O<sub>2</sub>-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H<sub>2</sub>O<sub>2</sub> production (Fig. 2). |
Revision as of 10:05, 11 January 2018
MiPNet18.06 Amplex-Yeast
O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. |
» »Versions
Oroboros (2015-06-15) Mitochondr Physiol Network
Abstract: Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2015) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(04):1-4
Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the O2k-Fluorescence LED2-Module. Extracellular H2O2-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H2O2 production (Fig. 2).
- >> Product: Oroboros O2k, O2k-Catalogue
• O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom
Labels: MiParea: Respiration
Organism: Saccharomyces cerevisiae, Fungi
Preparation: Intact cells
Regulation: Oxygen kinetics, Uncoupler Coupling state: ROUTINE, ET
HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-Demo, O2k-MultiSensor
See also
- A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. - »Bioblast link«