MiPNet19.05 IOC90 Bolzano IT

Bolzano IT, 2014 May 27. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC90 at ISMM2014.

» O2k-Workshop

OROBOROS (2014-05-27) Mitochondr Physiol Network

Abstract:

90th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry.

Info and preliminary programme: MiPNet19.05.pdf

• http://www.ismm2014.org/GeneralInformation/Pages/News.aspx

• O2k-Network Lab: AT Innsbruck OROBOROS

Labels:

Stress:Hypoxia, Temperature 

HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

ORO, IOC, 2014, Next 

Workshop and Conference

• ISMM2014 World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31

Date and time of O2k-Workshop

TUESDAY 2014-05-27 / 14.00 - 17.30

Seminar room 8

The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H₂O₂ production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’.

• More details? Consult »Hypothermia-Network: Mitochondria in the Cold.

Lecturers and tutors

Gnaiger Erich, CEO; Fontana-Ayoub Mona; Biomedical assistant; Fleischmann Sandra; Administrative assistant

• OROBOROS INSTRUMENTS

Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme)

• Gnaiger Erich, AT - Boushel Robert C, SE - Chicco Adam J, US - Murray Andrew J, UK - Horscroft JA, UK

Round table, will include additional keynote statements by

• Hermann Brugger, IT - Giacomo Strapazzon, IT - ad hoc participants

Recommended reading

• O2k-Publications: Hypoxia
• O2k-Publications: Oxygen kinetics
• O2k-Publications: Temperature

O2k-Core Manual

»O2k-Core Manual.pdf

SUIT protocols for high-resolution respirometry

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. »Bioblast Access

• Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. »Bioblast Access

HRR and O2k-Fluorometry

• »Manual: O2k-Fluorescence LED2-Module
• Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H₂O₂ production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. »Open Access
• »O2k-Fluorometry

Mitochondrial pathways

Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. »Open Access - handout to O2k-Workshop participants.

O2k-Workshops are listed as MitoGlobal Events.

• This workshop is a component of the K-Regio project MitoCom.