MiPNet19.05 IOC90 Bolzano IT: Difference between revisions
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The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H<sub>2</sub>O<sub>2</sub> production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’. | The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H<sub>2</sub>O<sub>2</sub> production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’. | ||
* ''More details? Consult | * ''More details? Consult'' »[[Hypothermia-Network: Mitochondria in the Cold]]. | ||
=== Lecturers and tutors === | === Lecturers and tutors === | ||
: [[File:Gnaiger Erich.jpg|80px|Erich Gnaiger]] [[File:Mona.jpg|80px|Fontana-Ayoub Mona]] [[File:FleischmannS.jpg|80px|Fleischmann Sandra]] | : [[File:Gnaiger Erich.jpg|80px|Erich Gnaiger]] [[File:Mona.jpg|80px|Fontana-Ayoub Mona]] [[File:FleischmannS.jpg|80px|Fleischmann Sandra]] | ||
Revision as of 15:00, 19 May 2014
 Bolzano IT, 2014 May 27. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC90 at ISMM2014. |
OROBOROS (2014-05-27) Mitochondr Physiol Network
Abstract:
90th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry.
Info and preliminary programme: MiPNet19.05.pdf
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels:
Stress:Hypoxia, Temperature
HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
ORO, IOC, 2014, Next
Workshop and Conference
- ISMM2014 World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31
Date and time of O2k-Workshop
- TUESDAY 2014-05-27 / 14.00 - 17.30
- Seminar room 8
The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H2O2 production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’.
- More details? Consult »Hypothermia-Network: Mitochondria in the Cold.
Lecturers and tutors
Gnaiger Erich, CEO;
Fontana-Ayoub Mona; Biomedical assistant;
Fleischmann Sandra; Administrative assistant
Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme)
- Gnaiger Erich, AT - Boushel Robert C, SE - Chicco Adam J, US - Murray Andrew J, UK - Horscroft JA, UK
Round table, will include additional keynote statements by
- Hermann Brugger, IT - Giacomo Strapazzon, IT - ad hoc participants
Recommended reading
O2k-Core Manual
SUIT protocols for high-resolution respirometry
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. »Bioblast Access
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. »Bioblast Access
HRR and O2k-Fluorometry
- »Manual: O2k-Fluorescence LED2-Module
- Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. »Open Access
- »O2k-Fluorometry
Mitochondrial pathways
Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. »Open Access - handout to O2k-Workshop participants.
O2k-Workshops are listed as MitoGlobal Events.
- This workshop is a component of the K-Regio project MitoCom.
