MiPNet19.05 IOC90 Bolzano IT
 Bolzano IT, 2014 May 27. Applied Workshop - High-resolution respirometry and mitochondrial function under hypoxia: overview, demo experiment and discussion. IOC90 at ISMM2014. |
OROBOROS (2014-05-27) Mitochondr Physiol Network
Abstract:
90th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry.
Info and preliminary programme: MiPNet19.05.pdf
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels:
Stress:Hypoxia, Temperature
HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
ORO, IOC, 2014, Next
Conference
- ISMM2014 World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31
O2k-Workshop
- TUESDAY 2014-05-27 / 14.00 - 17.30
- Seminar room 8
The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H2O2 production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’.
- More details? Consult »Hypothermia-Network: Mitochondria in the Cold.
Introduction
14:00 – 14:45 Introduction: high-resolution respirometry and mt-function in extreme environments
Gnaiger Erich, CEO;
Fontana-Ayoub Mona; Biomedical assistant;
Fleischmann Sandra; Administrative assistant;
Laner Verena; Project manager and scientific assistant
- OROBOROS INSTRUMENTS - General introduction into the Oxygraph-2k and high-resolution respirometry, O2k-Demo experiment on mitochondrial respiratory function under hypoxic steady-state (O2k with module Titration-Injection microPump).
Demo experiment
14:45 – 15:30 Demo experiment: mitochondrial respiration and H2O2 production under hypoxia
15:30 – 16:00 Coffee break
Round table: Hypoxia and mitochondrial function - practical experience
16:00 – 16.45 Round table discussion: Experience with mitochondrial respiration taking the Oxygraph-2k to >4,000 m
Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function.
- Gnaiger Erich, AT - Boushel Robert C, SE - Chicco Adam J, US - Murray Andrew J, UK - Horscroft JA, UK
Round table: Hypothermia and mitochondrial function - perspectives
16:45 – 17:00 Round table discussion: Clinical Hypothermia and Mitochondrial Function
Keynote statements by
- Strapazzon Giacomo, IT - Malacrida Sandro, IT - and ad hoc participants
Registration
- Visit us at the OROBOROS INSTRUMENTS exhibition booth from Monday to Wednesday, May 26 to 28.
- Here you can register for the OROBOROS O2k-Workshop until Tuesday morning, May 27. Free registration for ISMM participants.
Recommended reading
O2k-Core Manual
SUIT protocols for high-resolution respirometry
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. »Bioblast Access
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. »Bioblast Access
HRR and O2k-Fluorometry
- »Manual: O2k-Fluorescence LED2-Module
- Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. »Open Access
- »O2k-Fluorometry
Mitochondrial pathways
Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. »Open Access - handout to O2k-Workshop participants.
O2k-Workshops are listed as MitoGlobal Events.
- This workshop is a component of the K-Regio project MitoCom.
