MiPNet24.10 H2O2 flux analysis: Difference between revisions

Latest revision as of 08:55, 22 October 2021

MiPNet24.10 H2O2 flux analysis

Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4

» »Versions

Oroboros (2021-10-22) Mitochondr Physiol Network

Abstract: Komlodi T, Cardoso LHD, Gnaiger E (2021) Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4. Mitochondr Physiol Network 24.10(03):1-5.

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

Further details: » MitoPedia: O2k-Open Support

• O2k-Network Lab: AT_Innsbruck_Oroboros

Acknowledgements

Labels: MiParea: Respiration, Instruments;methods

HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol

O2k-MultiSensor, MitoFitPublication, SUIT-013, SUIT-013 AmR ce D023, SUIT-009, SUIT-009 AmR mt D021, SUIT-009 AmR pce D019, SUIT-018, SUIT-018 AmR mt D031, SUIT-006 AmR mt D048, SUIT-003 AmR ce D017, SUIT-003 AmR ce D058, O2k-Analysis, DatLab, DL7.4

@@ Line 2: / Line 2: @@
 {{Publication
 |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4
-|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/5/57/MiPNet24.10_H2O2 flux analysis with DatLab 7.4.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:MiPNet20.14_AmplexRed_H2O2-production.pdf Versions]
+|info=[[File:PDF.jpg|100px|link=https://wiki.oroboros.at/images/b/ba/MiPNet24.10_H2O2_analysis_template.pdf|Bioblast pdf]] »[https://www.bioblast.at/index.php/File:MiPNet24.10_H2O2_analysis_template.pdf Versions]
 |authors=Oroboros
-|year=2019-08-01
+|year=2021-10-22
 |journal=Mitochondr Physiol Network
-|abstract='''Komlodi T, Iglesias-Gonzalez J, Cardoso LHD, Sobotka O, Gnaiger E (2019) Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4. Mitochondr Physiol Network 24.10(01):1-5.'''
+|abstract=Komlodi T, Cardoso LHD, Gnaiger E (2021) Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4. Mitochondr Physiol Network 24.10(03):1-5.
+{{MiPNet pdf page linking to MitoPedia}}
-[[High-resolution respirometry]] is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: [[O2k-Publications:_O2k-Fluorometry_-_Fluorophores|H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels]].
-Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
-# Quality control of preparations by respirometric performance.
-# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores.
-# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
-# Additional information is acquired for a limited amount of sample.
-# Oxygen dependence of parameters can be studied.
-In the publication by [[Krumschnabel_2015_Methods_Mol_Biol|Krumschnabel et al. 2015]] we introduce the O2k-Fluo LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H<sub>2</sub>O<sub>2</sub> production of mouse brain mitochondria. The paper by [[Makrecka-Kuka_2015_Biomolecules|Makrecka-Kuka et al. 2015]] focuses on the application of the Amplex red assay for determining H<sub>2</sub>O<sub>2</sub> production in three different models of mitochondrial preparations.
-'''O2k-Publication''':
-[[Image:O2k-Publications.jpg|50px|link=O2k-Publications:_Topics|O2k-Publications in the MiPMap]]
-Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. [[Krumschnabel 2015 Methods Mol Biol |»Bioblast link«]]
-[[Image:O2k-Publications.jpg|50px|left|link=O2k-Publications:_Topics|O2k-Publications in the MiPMap]]
-Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. [[Makrecka-Kuka 2015 Biomolecules| »Bioblast link«]]
 |mipnetlab=AT_Innsbruck_Oroboros
 }}
+'''Acknowledgements'''
+[[File:Template NextGen-O2k.jpg|left|400px|link=NextGen-O2k]]
+<br/>
+<br/>
+<br/>
+<br/>
+<br/>
 {{Labeling
 |area=Respiration, Instruments;methods
 |instruments=Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
-|additional=O2k-Demo, O2k-MultiSensor, MitoFitPublication, SUIT-013, SUIT-013 AmR ce D023, SUIT-009, SUIT-009 AmR mt D021, SUIT-009 AmR pce D019, SUIT-018, SUIT-018 AmR mt D031, SUIT-006 AmR mt D048, SUIT-003 AmR ce D017, SUIT-003 AmR ce D058,
+|additional=O2k-MultiSensor, MitoFitPublication, SUIT-013, SUIT-013 AmR ce D023, SUIT-009, SUIT-009 AmR mt D021, SUIT-009 AmR pce D019, SUIT-018, SUIT-018 AmR mt D031, SUIT-006 AmR mt D048, SUIT-003 AmR ce D017, SUIT-003 AmR ce D058, O2k-Analysis, DatLab, DL7.4
 }}
-{{Keywords H2O2}}