Difference between revisions of "MiR06Cr"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=MiR07 | |abbr=MiR07 | ||
|description=Mitochondrial respiration medium, ''' | |description=Mitochondrial respiration medium, '''MiR06Cr''', developed for oxygraph incubations of mitochondrial preparations - ''[[permeabilized muscle fibre]]s''. | ||
MiR06Cr = [[MiR06]] + 20 mM [[Creatine|creatine]]. | |||
|info=[[MiPNet14.13]] | |info=[[MiPNet14.13]] | ||
}} | }} | ||
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|mitopedia topic=Medium | |mitopedia topic=Medium | ||
}} | }} | ||
The '''oxygen solubility factor''' of MiR06Cr is the same as for [[MiR05]] and [[MiR05Cr]] = '''0.92'''. | |||
'''Rationale:''' as for [[MiR06]] (the presence of catalase is protective, allows for reoxygenations with H2O2 injections, but prevents simultaneous measurement of H2O2 production), and less ADP needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect. | |||
Revision as of 07:30, 18 July 2012
- high-resolution terminology - matching measurements at high-resolution
MiR06Cr
Description
Mitochondrial respiration medium, MiR06Cr, developed for oxygraph incubations of mitochondrial preparations - permeabilized muscle fibres.
MiR06Cr = MiR06 + 20 mM creatine.
Abbreviation: MiR07
Reference: MiPNet14.13
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
The oxygen solubility factor of MiR06Cr is the same as for MiR05 and MiR05Cr = 0.92.
Rationale: as for MiR06 (the presence of catalase is protective, allows for reoxygenations with H2O2 injections, but prevents simultaneous measurement of H2O2 production), and less ADP needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect.
