Difference between revisions of "MiR06Cr"
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=MiR06Cr | ||
|description=Mitochondrial respiration medium, '''MiR06Cr''', developed for oxygraph incubations of mitochondrial preparations - ''[[permeabilized muscle fibre]]s''. Β | |description=Mitochondrial respiration medium, '''MiR06Cr''', developed for oxygraph incubations of mitochondrial preparations - ''[[permeabilized muscle fibre]]s''. Β | ||
Revision as of 07:31, 18 July 2012
Description
Mitochondrial respiration medium, MiR06Cr, developed for oxygraph incubations of mitochondrial preparations - permeabilized muscle fibres.
MiR06Cr = MiR06 + 20 mM creatine.
Abbreviation: MiR06Cr
Reference: MiPNet14.13
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
The oxygen solubility factor of MiR06Cr is the same as for MiR05 and MiR05Cr = 0.92.
Rationale: as for MiR06 (the presence of catalase is protective, allows for reoxygenations with H2O2 injections, but prevents simultaneous measurement of H2O2 production), and less ADP needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect.