Difference between revisions of "MiR06Cr"
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The '''oxygen solubility factor''' of MiR06Cr is the same as for [[MiR05]] and [[MiR05Cr]] = '''0.92'''. | * For preparation of MiR06Cr and application in HRR, see [[MiPNet14.13 Medium-MiR06]]. | ||
* The '''oxygen solubility factor''' of MiR06Cr is the same as for [[MiR05]] and [[MiR05Cr]] = '''0.92'''. | |||
'''Rationale:''' as for [[MiR06]] (the presence of catalase is protective, allows for reoxygenations with H2O2 injections, but prevents simultaneous measurement of H2O2 production), and less ADP needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect. | '''Rationale:''' as for [[MiR06]] (the presence of catalase is protective, allows for reoxygenations with H2O2 injections, but prevents simultaneous measurement of H2O2 production), and less ADP needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect. | ||
Revision as of 08:44, 18 July 2012
- high-resolution terminology - matching measurements at high-resolution
MiR06Cr
Description
Mitochondrial respiration medium, MiR06Cr, developed for oxygraph incubations of mitochondrial preparations - permeabilized muscle fibres.
MiR06Cr = MiR06 + 20 mM creatine.
Abbreviation: MiR06Cr
Reference: MiPNet14.13
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
- For preparation of MiR06Cr and application in HRR, see MiPNet14.13 Medium-MiR06.
- The oxygen solubility factor of MiR06Cr is the same as for MiR05 and MiR05Cr = 0.92.
Rationale: as for MiR06 (the presence of catalase is protective, allows for reoxygenations with H2O2 injections, but prevents simultaneous measurement of H2O2 production), and less ADP needed in permeabilized muscle fibres. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect.
