MitoKit-CII/Succinate-nv

high-resolution terminology - matching measurements at high-resolution

MitoKit-CII/Succinate-nv

Description

MitoKit-CII/Succinate-nv (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug (permeable succinate; Snv) that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing succinate in the intracellular space. NeuroVive #: NV118

Abbreviation: Snv

Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun

MitoPedia topics: Media for respirometry 

Application in HRR

Succinate-nv: 25 mg, MW=262.2 g.mol^-1

Preparation of 1 M stock solution:

1. Dissolve 25 mg Snv in 95.3 µL DMSO.

Preparation of 100 mM stock solution of Snv (from the 1 M stock):

1. Dilute 95.3 µL 1 M stock solution with 857.7 µL DMSO.
2. Divide into 50 µL portions.
3. Store up to 6 months at -20 °C or up to 1 week at room temperature.

» O2k manual titrations

• Event name: Snv
• Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
• Final concentration: 50 µM steps.
• Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.