Myxothiazol: Difference between revisions

Revision as of 10:26, 11 September 2020

high-resolution terminology - matching measurements at high-resolution

Myxothiazol

Description

Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII also inhibits CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome b_L) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.

Abbreviation: Myx

Reference: MiPNet09.12

Application in HRR

Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C₂₅H₃₃N₃O₃S₂); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.

Caution: Very toxic.

Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol):

Weigh 1 mg of myxothiazol into a small glass vial.
Add 2.05 mL ethanol.
Divide into 0.2 mL (use glass vials).
Store at -20 °C.

»O2k manual titrations MiPNet09.12

Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-chamber).
Final concentration: 0.5 µM.

Compare

Antimycin A is less expensive than Myx but induces higher ROS production.

MitoPedia topics: Inhibitor

@@ Line 8: / Line 8: @@
 == Application in [[HRR]] ==
-'''Myx: Myxothiazol''' (from ''Myxobacterium myxococcus fulvus'', ~95%, HPLC), C<sub>25</sub>H<sub>33</sub>N<sub>3</sub>O<sub>3</sub>S<sub>2</sub>); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.
+:::'''Myx: Myxothiazol''' (from ''Myxobacterium myxococcus fulvus'', ~95%, HPLC), C<sub>25</sub>H<sub>33</sub>N<sub>3</sub>O<sub>3</sub>S<sub>2</sub>); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.
-<span style="color:#8B008B"> '''Caution:''' Very toxic.</span>
+::::<span style="color:#8B008B"> '''Caution:''' Very toxic.</span>
+::::'''Preparation of 1 mM storage and stock solution''' (dissolved in 99.9% ethanol):
+::::# Weigh 1 mg of myxothiazol into a small glass vial.
+::::# Add 2.05 mL ethanol.
+::::# Divide into 0.2 mL (use glass vials).
+::::# Store at -20 °C.
-'''Preparation of 1 mM storage and stock solution''' (dissolved in 99.9% ethanol):
-:# Weigh 1 mg of myxothiazol into a small glass vial.
+:::»'''O2k manual titrations'''  [[MiPNet09.12]]
-:# Add 2.05 mL ethanol.
+::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-chamber).
-:# Divide into 0.2 mL (use glass vials).
+::::* Final concentration: 0.5 µM.
-:# Store at -20 °C.
-'''O2k manual titrations'''  [[MiPNet09.12]]
-:* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-chamber).
-:* Final concentration: 0.5 µM.