Myxothiazol: Difference between revisions

Revision as of 09:18, 12 October 2015

high-resolution terminology - matching measurements at high-resolution

Myxothiazol

Description

Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII inhibits also CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome b_L) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.

Abbreviation: Myx

Reference: MiPNet09.12

MitoPedia topics: Inhibitor

Application in HRR

Myx: Myxothiazol (from Myxobacterium myxococcus fulvus, ~95%, HPLC), C₂₅H₃₃N₃O₃S₂); Sigma T 5580, 1 mg, store at 4 °C: FW = 487.7. Available again from Sigma with this new product number. The old product number was M 5779.

Caution: Very toxic.

Preparation of 1 mM storage and stock solution (dissolved in 99.9% ethanol):

Weigh 1 mg of myxothiazol into a small glass vial.
Add 2.05 ml ethanol.
Divide into 0.2 ml (use glass vials).
Store at -20 °C.

Oxygraph-2k manual titrations MiPNet09.12

Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
Final concentration: 0.5 µM.

Compare

Antimycin A is less expensive than Myx but induces higher ROS production.

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Myx
-|description='''Myxothiazol''' is an inhibitor of [[Complex III|CIII]] (inhibits also [[Complex I|CI]]; G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome ''b''<sub>L</sub>) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
+|description='''Myxothiazol''', Myx, is an inhibitor of [[Complex III]] (CIII). CIII inhibits also [[Complex I|CI]] (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome ''b''<sub>L</sub>) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.
 |info=[[MiPNet09.12]]
 }}
@@ Line 17: / Line 17: @@
 '''Preparation of 1 mM storage and stock solution''' (dissolved in 99.9% ethanol):
-::1) Weigh 1 mg of myxothiazol into a small glass vial.
+:# Weigh 1 mg of myxothiazol into a small glass vial.
-::2) Add 2.05 ml ethanol.
+:# Add 2.05 ml ethanol.
-::3) Divide into 0.2 ml (use glass vials).
+:# Divide into 0.2 ml (use glass vials).
-::4) Store at -20 °C.
+:# Store at -20 °C.
 '''Oxygraph-2k manual titrations'''  [[MiPNet09.12]]
-::* Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
+:* Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
-::* Final concentration: 0.5 µM.
+:* Final concentration: 0.5 µM.
 == Compare ==
-* [[Antimycin A]]
+* [[Antimycin A]] is less expensive than Myx but induces higher ROS production.