O2k-Videosupport: Difference between revisions

Revision as of 15:09, 27 April 2021

O2k-Videosupport

» The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.

» This cannot replace a careful study of the O2k-Manual.

O2k-start (DatLab 7/Series H)

MiPNet22.11 O2k-FluoRespirometer manual

O2k ready to use

A1 Oroboros flash drive and DatLab 7 installation

A2 Insert O2k Chamber

A3 Insert OroboPOS

A4 O2k-chamber volume calibration

A5 DL7 Start DatLab7

FluoRespirometry

Smart Fluo-Sensor assembly

Exchanging a filter set of the smart-fluo sensor

H₂O₂ flux analysis with DatLab 7.4

O2k-start (DatLab 6/Series G)

MiPNet19.18A O2k-Series G: Start

Insert O2k-Chamber

Insert OroboPOS

O2k-Chamber volume calibration

O2k ready to use

POS-service and Chamber-service (all series)

POS-service

MiPNet19.18B POS-service

OroboPOS from Perspex housing

Disassembly of OroboPOS

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration

Chamber-service

Chamber service Insert O2k Chamber

Chamber service O2k Chamber volume calibration

DatLab 7.4

How to find a DL-Protocol (DLP)

How to perform an experiment with a SUIT DL-Protocol (DLP)

O₂ flux analysis with DatLab 7.4

Export DL-Protocol User (*.DLPU)

O2k-Mitochondrial preparations

MiPNet17.02 PBI-Shredder manual

MiPNet14.14 PermeabilizedFiberPreparation

MiPNet17.03 Shredder vs Fibers

Shredder tissue homogenate

Fiber permeabilization

Addition of biological sample to the respirometer chamber

Q-Module (NextGen-O2k - DatLab 7)

Q-service

Assembly of the reference electrode

NG Polishing glassy carbon and platinum electrodes

NG O2k-Chamber volume calibration with Q-Sensor

Q-experiments

NG Q-experiments

Cleaning Q-Sensors

NG Cleaning after cyclic voltammetry

NG Cleaning after Q-experiments

@@ Line 31: / Line 31: @@
 [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7]
+=== '''FluoRespirometry''' ===
+[[Image:PlayVideo.jpg|50px|link= ]] Smart Fluo-Sensor assembly
+[[Image:PlayVideo.jpg|50px|link= ]] Exchanging a filter set of the smart-fluo sensor
+[[Image:PlayVideo.jpg|50px|link= ]] H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4
 </div>
@@ Line 77: / Line 86: @@
 [[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ DL7 Insert OroboPOS]
+[[Image:PlayVideo.jpg|50px|link= ]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration
 ---------
@@ Line 128: / Line 140: @@
 [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be]] [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be  Fiber permeabilization]
+[[Image:PlayVideo.jpg|50px|link= ]] Addition of biological sample to the respirometer chamber
 </div>
@@ Line 172: / Line 185: @@
-[[File:Expand.png|right|45px |Click to expand or collaps]]
-<div class="toccolours mw-collapsible">
-== Exclusive material for O2k-Workshop ==
-<div class="mw-collapsible-content">
-* '''O2k Quality Control'''
-[[Image:PlayVideo.jpg|50px|link= ]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration
----------
-* '''O2k-Mitochondrial preparations'''
-[[Image:PlayVideo.jpg|50px|link= ]] Addition of biological sample to the respirometer chamber
----------
-* '''FluoRespirometry'''
-[[Image:PlayVideo.jpg|50px|link= ]] Smart Fluo-Sensor assembly
-[[Image:PlayVideo.jpg|50px|link= ]] Exchanging a filter set of the smart-fluo sensor
-[[Image:PlayVideo.jpg|50px|link= ]] H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4
-</div>
-</div>
 </div>
 </div>
 <br/>