Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "O2k-Workshops"

From Bioblast
(30 intermediate revisions by 9 users not shown)
Line 1: Line 1:
{{Product
{{OROBOROS header page name}}
|description='''OROBOROS O2k-Workshops''' are organized as international oxygraph courses (IOC) on [[high-resolution respirometry]] since 1991. These range from four full-day training courses to conference lunch presentations of the OROBOROS '''[[Oxygraph-2k]] and [[O2k-Fluorometer]]'''.
:::: '''Oroboros O2k-Workshops''' are organized as international oxygraph courses (IOC) on [[high-resolution respirometry]] since 1991. These range from four full-day training courses to conference lunch presentations of the [[Oroboros O2k]] and particularly the [[O2k-FluoRespirometer]]'''.
|product id=7'''####'''
|product type=O2k, Catalogue
|info=[[OROBOROS_Events#Next_O2k-Workshops |Next OROBOROS O2k-Workshops]]
|product image=[[Image:O2k-Workshops.png|right|180px]]
}}
__TOC__


== General contents of O2k-Workshops ==
:::* [[Image:O2k-Workshops.png|right|180px]] [[Oroboros_Events#Next_O2k-Workshops |Next Oroboros O2k-Workshops]]


* Mitochondrial and cellular respiratory physiology; new challenges for high instrumental performance.
* [[O2k high-resolution respirometry]].
* Optimum system design - the OROBOROS [[Oxygraph-2k]].
* [[DatLab]]: real-time recording of oxygen concentration and flux; linear slope versus oxygen flux as a function of time.
* DatLab: the specialized software for high-resolution respirometry; high resolution calibrations.
* Low oxygen and measurement of cellular oxygen consumption – pushing the limits of detection.


__TOC__


== General contents of O2k-Workshops ==


'''O2k and TIP2k: Real-time instrumental performance'''
::::* Mitochondrial and cellular respiratory physiology; new challenges for high instrumental performance.
::::* [[High-resolution respirometry]].
::::* Optimum system design - the [[Oroboros O2k]].
::::* [[DatLab]]: the specialized software for high-resolution respirometry and high-resolution calibrations with real-time recording.
::::* Low oxygen and measurement of cellular oxygen consumption – pushing the limits of detection.


''' O2k and TIP2k: Real-time instrumental performance'''
::::* [[MiPNet14.06 Instrumental O2 background |Instrumental background oxygen flux]]: measurement and correction as a function of ''p''<sub>O2</sub>.
::::* High-resolution of [[Substrate-uncoupler-inhibitor titration|respiratory flux at various steady-states, SUIT protocols]].
<!-- ::::* O2k-MultiSensor applications: TPP<sup>+</sup>, pH, NO  -->
::::* [[Titration-Injection microPump]] (TIP2k): automatic titrations.
::::* O2k and TIP2k: instrumental maintenance.
::::* Conceptual and methodological advantages of measurement at physiological low levels of oxygen.


* [[Instrumental background oxygen flux]]: measurement and correction as a function of ''p''<sub>O2</sub>.
''' [[OroboPOS| Polarographic oxygen sensor (POS)]] and O2k service'''
* High-resolution of [[Substrate-uncoupler-inhibitor titration |respiratory flux at various steady-states]].
::::* Cleaning of anode and cathode.
* O2k-MultiSensor applications: TPP+, pH, NO
::::* Electrolyte and membrane application.
* [[Titration-Injection microPump]] (TIP2k): automatic titrations.
* Conceptual and methodological advantages of measurement at physiological low levels of oxygen.


'''[[O2k-Fluo Smart-Module]]'''




'''[[OroboPOS| Polarographic oxygen sensor (POS)]] and O2k service'''
== Next O2k-Workshops ==
 
 
* Cleaning of anode and cathode.
* Electrolyte and membrane application.
* O2k and TIP2k: instrumental maintenance.
 


 
{{#ask:[[Category:Publications]] [[Additional label::IOC]] [[Additional label::Next]]
== Next O2k-Workshops ==
|?Was published in year=Date
:» [[OROBOROS_Events#Next_O2k-Workshops| More details]]
|?Has title=Location - Country - IOC
|format=broadtable
|limit=5000
|offset=0
|sort=Was published in year
|order=ascending
}}
:» [[Oroboros_Events| More details]]




Line 56: Line 57:


== Formats ==
== Formats ==
::::» '''[[MiPNet26.07 Installation and startup support session|Virtual Oroboros Installation and startup support session]]'''


::::» [[IOC Schroecken |'''O2k-Workshops in Schroecken (Austria)''']]


=== O2k-Workshop: HRR and O2k-Fluorometry ===
::::» '''[[O2k-Coaching Days Innsbruck]]'''


The O2k-Workshop on HRR and Fluorometry provides a detailed introduction to the OROBOROS Oxygraph-2k ([[O2k-Core]]), overview presentations on [[O2k high-resolution respirometry]] (HRR), and applications in mitochondrial physiology. An introduction to O2k-Fluorometry is integrated into the programme.
::::» '''[[MiPNet25.16 Virtual O2k-Workshop HRR| Virtual O2k-Workshops]]'''


:::: O2k-Workshops have become world-famous. They are offered on a regular basis in Austria and in other countries in cooperation with [[O2k-Network Labs]].


The O2k-Workshop programme is complemented by lectures on mitochondrial physiologoy (general topics; [[O2k-Protocols]]). Short presentations of 'Hot topics in Mitochondrial Physiology' provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.


=== O2k-Workshop: HRR and the O2k-FluoRespirometer ===


=== O2k-Workshop: O2k-Fluorometry  ===
:::: The O2k-Workshop on high-resolution respirometry provides a detailed introduction to the [[Oroboros O2k]], overview presentations on [high-resolution respirometry]] (HRR), and applications in mitochondrial physiology.


The O2k-Fluorometry Workshop is integrated into the general O2k-Workshop, introducing fluorometry combined with high-resolution respirometry (HRR). Following a short introduction to HRR, the O2k-Fluorescence LED2-Module is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production [[Amplex red| (Amplex red)]], mt-membrane potential [[Safranin|(Safranin)]], ATP production [[Magnesium green| (Mg green)]], and Ca2+ [[Calcium green| (Ca green)]].
:::: The O2k-Workshop program is complemented by lectures on mitochondrial physiology (general topics; [[O2k-Protocols]]). Short presentations of 'O2k perspectives' provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.




Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of 'Hot topics in Mitochondrial Physiology'.
=== O2k-Workshop: Advanced O2k-FluoRespirometry  ===


:::: The Advanced O2k-FluoRespirometry Workshop is focused on fluorometry combined with respirometry. High-resolution respirometry (HRR) is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production [[Amplex red| (Amplex red)]], mt-membrane potential ([[Safranin]]), ATP production ([[Magnesium green]]), and Ca<sup>2+</sup> ([[Calcium green]]). Further O2k-MultiSensor applications are covered in these specific O2k-Workshops (e.g. [[IOC95| IOC95)]].


Information on O2k-Fluorometry, with resolution of hydrogenperoxide in the nanomolar range: [[O2k-Fluo LED2-Module| O2k-Fluorescence LED2-Module]].
:::: Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of 'O2k perspectives'.


 
:::: Information on O2k-FluoRespirometry, with resolution of hydrogenperoxide in the nanomolar range: [[O2k-FluoRespirometer]].
Moreover, O2k-Workshops with a special focus on O2k-Fluorometry are offered (e.g. [[IOC95| IOC95)]]. Further O2k-MultiSensor applications are covered in these specific O2k-Workshops.




=== O2k-Workshop: TPP/ ISE ===
=== O2k-Workshop: TPP/ ISE ===


The O2k-Workshop TPP/ISE is customized for participants interested in O2k-MultiSensor applications, particularly ion selective electrodes (ISE) for measurement of [http://www.oroboros.at/index.php?membrane-potential| mitochondrial membrane potential] (TPP+, TPMP+) and Ca2+. It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP+ to mt-membrane potential) constitutes a major topic.
:::: The O2k-Workshop TPP/ISE is customized for participants interested in O2k-MultiSensor applications, particularly ion selective electrodes (ISE) for measurement of [[mitochondrial membrane potential]] (TPP+, TPMP+) and Ca<sup>2+</sup>. It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP+ to mt-membrane potential) constitutes a major topic.
 
 
According to the interest of participants, other O2k-MultiSensor applications can be discussed and demonstrated:


* [[Calcium electrode| Measurement of Ca2+]], calibration and calculation of free Ca2+ in buffered solutions (O2k-TPP+ and Ca2+ ISE-Module)
:::: According to the interest of participants, other O2k-MultiSensor applications can be discussed and demonstrated:
* [[O2k-pH ISE-Module|Acidification rate (pH)]]
::::* [[Calcium electrode| Measurement of Ca<sup>2+</sup>]], calibration and calculation of free Ca<sup>2+</sup> in buffered solutions ([[O2k-TPP+ ISE-Module]])
* [http://www.oroboros.at/index.php?id=o2k-fluorescence| O2k-Fluorescence]
::::* [[O2k-pH ISE-Module|Acidification rate (pH)]]
* [http://www.oroboros.at/index.php?id=no-oxygen| Nitric Oxide (NO)]
::::* [[O2k-FluoRespirometer]]
::::* [[O2k-NO Amp-Module| Nitric Oxide (NO)]]






=== O2k-Workshops in O2k-Network Labs  ===
=== O2k-Workshops in O2k-Network Labs  ===
:» [[O2k-Workshops in O2k-Network Labs |More details]]
::::» [[O2k-Workshops in O2k-Network Labs |More details]]
 


<gallery mode=packed widths="252px" heights="216px" perrow=4>
<gallery mode=packed widths="252px" heights="216px" perrow=4>
Line 112: Line 113:
== Abstracts ==
== Abstracts ==


Abstracts are welcome for short presentations, providing participants for an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.
:::: Abstracts are welcome for short presentations, providing participants for an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.


Abstracts should be submitted to [mailto:[email protected] [email protected]]
:::: Abstracts should be submitted to [mailto:[email protected] [email protected]]


''' Instructions for IOC-Abstracts'''
:::: Abstracts  are welcome for short  presentations (O2k perspectives), providing participants an opportunity to present and discuss their work, including projects in progress or in the planning state, methodological details and conceptual aspects.


 
::::* '''Length of text''': Max. 500 words including up to three references (all authors, full title) and a figure legend. The abstract does not allow excess length of text. Additional information (further references etc.) may be added into the free text which will not be included in the printed version.
 
::::* You may edit your abstract on the website any time. However, please consider the '''deadline for the printed program'''.
'''Instructions for IOC-Abstracts'''
::::* Kindly submit your abstract by emailing it to us in a wordfile. We will then add your abstract to the bioblast wiki. Please let us know if you do not wish your abstract to be open access.
 
::::* '''Figures''': A figure is welcome. Please send us a '''figure''' (jpg or png) and your '''photo''' (jpg or png).
Abstracts  are welcome for short  presentations (hot topics in mitochondrial  physiology), providing participants an opportunity to present and discuss their work, including projects in progress or in the planning state, methodological details and conceptual aspects.
 
* '''Length of text''': Max. 500 words including up to three references (all authors, full title) and a figure legend. The abstract does not allow excess length of text. Additional information (further references etc.) may be added into the free text which will not be included in the printed version.
* You may edit your abstract on the website any time. However, please consider the '''deadline for the printed programme'''.
* Kindly submit your abstract by emailing it to us in a wordfile. We will then add your abstract to the bioblast wiki. Please let us know if you do not wish your abstract to be open access.
* '''Figures''': A figure is welcome. Please send us a '''figure''' (jpg or png) and your '''photo''' (jpg or png).




Line 141: Line 138:




== OROBOROS travel grant ==
== Oroboros travel grant ==


OROBOROS INSTRUMENTS offers travel grants for the support of scientists attending the O2k-Workshop on High-Resolution Respirometry (HRR).
:::: Oroboros Instruments offers travel grants for the support of scientists attending the O2k-Workshop on high-resolution respirometry.


'''Eligibility and Application'''
::: '''Eligibility and Application'''


* With the purchase of an O2k, you are eligible for receiving an OROBOROS Travel Grant.
::::* Depending on the O2k package you purchased, you may be eligible for receiving an Oroboros Travel Grant. Please contact Oroboros Instruments for further information.
* Specific academic OROBOROS Travel Grants are availble as start-up support. Scientists establishing a new lab in their first university position may apply by sending a letter of confirmation by their university.


'''General Information'''
::: '''General Information'''


* The number of OROBOROS Travel Grants is limited.  
::::* The number of Oroboros Travel Grants is limited.  
* OROBOROS Travel Grants are limited to one per research group.
::::* Oroboros Travel Grants are limited to one per research group.
* The selection of awardees is made by OROBOROS INSTRUMENTS.
::::* The selection of awardees is made by Oroboros Instruments.




== IOC recommended reading ==
== IOC recommended reading ==
:» [[IOC recommended reading| More details]]
::::» [[IOC recommended reading| More details]]


== Contact ==
== Contact ==


:» [[OROBOROS_Contact |OROBOROS INSTRUMENTS]]
::::» [[Oroboros contact |Oroboros Instruments]]
 
<gallery mode=packed widths="252px" heights="216px" perrow=4>
File:Ioc56 presentation.jpg
File:Ioc41 blue book.jpg
File:Ioc51 oxygraph tpp2.jpg
File:Ioc59 hands on titration.jpg
File:Ioc44 groupppic.jpg
File:Ioc46 mohnenfluch lunch.jpg
File:Ioc47 alpmuseum edwin.jpg
File:Ioc55 alpmuseum game.jpg
</gallery>

Revision as of 12:33, 11 July 2022

                



O2k-Workshops

Oroboros O2k-Workshops are organized as international oxygraph courses (IOC) on high-resolution respirometry since 1991. These range from four full-day training courses to conference lunch presentations of the Oroboros O2k and particularly the O2k-FluoRespirometer.


General contents of O2k-Workshops

  • Mitochondrial and cellular respiratory physiology; new challenges for high instrumental performance.
  • High-resolution respirometry.
  • Optimum system design - the Oroboros O2k.
  • DatLab: the specialized software for high-resolution respirometry and high-resolution calibrations with real-time recording.
  • Low oxygen and measurement of cellular oxygen consumption – pushing the limits of detection.

O2k and TIP2k: Real-time instrumental performance

Polarographic oxygen sensor (POS) and O2k service

  • Cleaning of anode and cathode.
  • Electrolyte and membrane application.

O2k-Fluo Smart-Module


Next O2k-Workshops

 DateLocation - Country - IOC
MiPNet28.11 IOC163 Schroecken AT2024-06-17
O2k-Workshops.png
Schroecken AT, 2024 Jun 17-22. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC163.
MiPNet28.13 IOC165 Innsbruck AT2024-09-02
O2k-Workshops.png
Innsbruck AT, 2024 Sep 02-04. EBEC2024 Satellite Oroboros O2k-Workshop: Mito&Chlora High-Resolution Respirometry and PhotoBiology, IOC165.
MiPNet28.12 IOC164 Schroecken AT2024-09-30
O2k-Workshops.png
Schroecken AT, 2024 Sep 30 - Oct 05. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC164.
» More details



Formats

» Virtual Oroboros Installation and startup support session
» O2k-Workshops in Schroecken (Austria)
» O2k-Coaching Days Innsbruck
» Virtual O2k-Workshops
O2k-Workshops have become world-famous. They are offered on a regular basis in Austria and in other countries in cooperation with O2k-Network Labs.


O2k-Workshop: HRR and the O2k-FluoRespirometer

The O2k-Workshop on high-resolution respirometry provides a detailed introduction to the Oroboros O2k, overview presentations on [high-resolution respirometry]] (HRR), and applications in mitochondrial physiology.
The O2k-Workshop program is complemented by lectures on mitochondrial physiology (general topics; O2k-Protocols). Short presentations of 'O2k perspectives' provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.


O2k-Workshop: Advanced O2k-FluoRespirometry

The Advanced O2k-FluoRespirometry Workshop is focused on fluorometry combined with respirometry. High-resolution respirometry (HRR) is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membrane potential (Safranin), ATP production (Magnesium green), and Ca2+ (Calcium green). Further O2k-MultiSensor applications are covered in these specific O2k-Workshops (e.g. IOC95).
Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of 'O2k perspectives'.
Information on O2k-FluoRespirometry, with resolution of hydrogenperoxide in the nanomolar range: O2k-FluoRespirometer.


O2k-Workshop: TPP/ ISE

The O2k-Workshop TPP/ISE is customized for participants interested in O2k-MultiSensor applications, particularly ion selective electrodes (ISE) for measurement of mitochondrial membrane potential (TPP+, TPMP+) and Ca2+. It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP+ to mt-membrane potential) constitutes a major topic.
According to the interest of participants, other O2k-MultiSensor applications can be discussed and demonstrated:


O2k-Workshops in O2k-Network Labs

» More details


Abstracts

Abstracts are welcome for short presentations, providing participants for an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.
Abstracts should be submitted to [email protected]

Instructions for IOC-Abstracts

Abstracts are welcome for short presentations (O2k perspectives), providing participants an opportunity to present and discuss their work, including projects in progress or in the planning state, methodological details and conceptual aspects.
  • Length of text: Max. 500 words including up to three references (all authors, full title) and a figure legend. The abstract does not allow excess length of text. Additional information (further references etc.) may be added into the free text which will not be included in the printed version.
  • You may edit your abstract on the website any time. However, please consider the deadline for the printed program.
  • Kindly submit your abstract by emailing it to us in a wordfile. We will then add your abstract to the bioblast wiki. Please let us know if you do not wish your abstract to be open access.
  • Figures: A figure is welcome. Please send us a figure (jpg or png) and your photo (jpg or png).



Oroboros travel grant

Oroboros Instruments offers travel grants for the support of scientists attending the O2k-Workshop on high-resolution respirometry.
Eligibility and Application
  • Depending on the O2k package you purchased, you may be eligible for receiving an Oroboros Travel Grant. Please contact Oroboros Instruments for further information.
General Information
  • The number of Oroboros Travel Grants is limited.
  • Oroboros Travel Grants are limited to one per research group.
  • The selection of awardees is made by Oroboros Instruments.


IOC recommended reading

» More details

Contact

» [email protected]
» Oroboros Instruments