From Bioblast
O2k-chamber
Description | O2k-Chamber: 16 mm inner diameter, Duran glass polished, with standard operation volume (V) of 2 cm3 (2 ml).
Two units of this item are standard components of the O2k-Assembly Kit (O2k-Core). The optical properties of Duran allow application in the O2k-Fluorometer (Duran optical properties). |
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Product ID | 23100-01 |
Type | O2k, O2k-Assembly Kit |
Link | MiPNet09.01 O2k-ParadigmShift |
Image |
A spare (third) O2k-Chamber may be added to the items ordered with the O2k-System. For dispatch of the O2k, the O2k-Chambers are shipped in O2k-Service Box.
O2k-technical support
Cleaning the glass chamber
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- Standard operating procedure - cleaning and storage of the O2k-Chamber: >> MiPNet19.03 O2k-cleaning and ISS
Chamber assembly
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- The assembly of the chamber is described in MiPNet19.18A_O2k-Start.
Possible problems
- Chamber leaking liquids (medium is leaking out of the chamber)
- Oxygen leak of the chamber (strongly negative intercept in the instrumental background test
- Unstable O2 signal
- Unexpected high or low O2 signal
Trouble shooting procedure
- If the problem is obviously related to the chamber proceed to solutions. If the problem manifests in a very low or zero raw signal, the possible contribution of chamber assembly to the problem is easily checked by doing the first step of Locating_a_problem#Remove_components: if a very low or zero raw signal changes to the expected values (see Raw signal the problem is the chamber assembly.
- Otherwise follow Locating a problem
- If the problem is not located on the POS or the POS connector try solving the problem by reassembling the chamber before concluding that the main unit is defect.
Solutions
- Disassemble the chamber by reversing the instructions in MiPNet19.18A O2k-Start.
- Check for visible damages on the glass chamber, on the POS seal tip and all o-rings (on chamber holders or on stoppers). If a damage is visible replace the part or consult OROBOROS Instruments. If there is a deposit visible in the glass chamber see Cleaning the glass chamber.
- Reassemble the chamber following exactly the description in MiPNet19.18A O2k-Start. Especially, use only small increments (not more than successive quarter turns of the POS holder) when lowering the chamber.
Chamber volume calibration
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- The volume calibration of the chamber is described in MiPNet19.18A_O2k-Start.
- Old model stopper: if you use blue Titanium stoppers: check the manual delivered with your O2k or contact OROBOROS INSTRUMENTS ([email protected]) for instructions.
Glass chamber stuck in the O2k
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Problem
- After removing OroboPOS-Connector, OroboPOS-Holder, and O2k-Chamber Holder from the O2k , the O2k-Chamber cannot be removed. It remains stuck even after washing with water.
Solution
- The reason for this problem is very likely medium that has been spilled and acts now as a glue between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 Β°C. You can try GENTLY to use the sensor holder (not the sensor connector) to try to push up the glass chamber (like you do during chamber assembly) - while the O2k is warm and with plenty of warm water. Since the sensors are removed you also can use e.g. 40Β° (or a little bit more) for the washing / removing attempts.