Ost 2015 Fatty Acid Oxidation O2k-Network Discussion Forum: Difference between revisions
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|authors=Ost M | |authors=Ost M | ||
|year=2015 | |year=2015 | ||
|abstract=Right now weΒ΄re using octanoyl carnitine (OC) when examining mouse skeletal muscle permeabilized fiber respiration. We ususally start with Malate (2mM) and OC (0.2mM), then add ADP (5mM) in order to assess fatty acid oxidative capacity. For Soleus muscle fibers we got an average respiration of 60 pmol/(s*mg fiber ww). Β | |abstract=Right now weΒ΄re using octanoyl carnitine (OC) when examining mouse skeletal muscle permeabilized fiber respiration. We ususally start with Malate (2mM) and OC (0.2mM), then add ADP (5mM) in order to assess fatty acid oxidative capacity. For Soleus muscle fibers we got an average respiration of 60 pmol/(s*mg fiber ww). | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Mouse | |||
|tissues=Skeletal muscle | |||
|preparations=Permeabilized tissue | |||
|substratestates=FAO | |substratestates=FAO | ||
|additional=Octanoylcarnitine | |additional=Octanoylcarnitine, Malate, ADP | ||
}} | }} |
Revision as of 10:07, 9 November 2015
Ost 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Ost M (2015)
Event:
Right now weΒ΄re using octanoyl carnitine (OC) when examining mouse skeletal muscle permeabilized fiber respiration. We ususally start with Malate (2mM) and OC (0.2mM), then add ADP (5mM) in order to assess fatty acid oxidative capacity. For Soleus muscle fibers we got an average respiration of 60 pmol/(s*mg fiber ww).
Labels:
Organism: Mouse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Octanoylcarnitine, Malate, ADP