Oxygen solubility factor: Difference between revisions
(add link to oxygen solubility) |
mNo edit summary |
||
(24 intermediate revisions by 6 users not shown) | |||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=F<sub>M</sub> | |abbr=''F''<sub>M</sub> | ||
|description=The '''oxygen solubility factor''' of the incubation medium, | |description=The '''oxygen solubility factor''' of the incubation medium, ''F''<sub>M</sub>, expresses the effect of the salt concentration on [[oxygen solubility]] relative to pure water. In mitochondrial respiration medium [[MiR05]], [[MiR05-Kit]] and [[MiR06]], ''F''<sub>M</sub> is 0.92 (determined at 30 and 37 °C) and in culture media is 0.89 (at 37 °C). ''F''<sub>M</sub> varies depending on the temperature and composition of the medium. To determine the FM based on the oxygen concentration, specific methods and equipment are needed (see references Rasmussen HN, Rasmussen UF 2003 in [https://wiki.oroboros.at/index.php/MiPNet06.03_POS-calibration-SOP MiPNet06.03]). For other media, ''F''<sub>M</sub> may be estimated using Table 4 in [https://wiki.oroboros.at/index.php/MiPNet06.03_POS-calibration-SOP MiPNet06.03]. For this purpose KCl based media can be described as "seawater" of varying salinity. The original data on sucrose and KCl-media (Reynafarje et al 1985), however, have been critizesed as artefacts and the ''F''<sub>M</sub> of 0.92 is suggested in the temperature range of 10 °C to 40 °C as for MiR05. | ||
|info=[[MiPNet06.03]] | |||
| | |info=[[MiPNet06.03 POS-calibration-SOP]] | ||
}} | |||
== Keywords == | |||
{{Keywords: Concentration and pressure}} | |||
{{Template:Keywords: Oxygen signal}} | |||
[[Image:MiPNet06.03_Table.jpg|right|frame|<sup>c</sup>O2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. ''F''<sub>M</sub> has been determined for [[MiR05]] at 30 °C and 37 °C, and is assumed to be identical at 25 °C, and identical in [[MiR06]].]] | |||
{{MitoPedia methods | |||
|mitopedia method=Respirometry | |||
}} | |||
{{MitoPedia O2k and high-resolution respirometry | |||
|mitopedia O2k and high-resolution respirometry=DatLab | |||
}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Medium | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments= | |instruments=Theory | ||
}} | }} | ||
[[Category:MitoPedia]] |
Latest revision as of 10:22, 1 May 2022
Description
The oxygen solubility factor of the incubation medium, FM, expresses the effect of the salt concentration on oxygen solubility relative to pure water. In mitochondrial respiration medium MiR05, MiR05-Kit and MiR06, FM is 0.92 (determined at 30 and 37 °C) and in culture media is 0.89 (at 37 °C). FM varies depending on the temperature and composition of the medium. To determine the FM based on the oxygen concentration, specific methods and equipment are needed (see references Rasmussen HN, Rasmussen UF 2003 in MiPNet06.03). For other media, FM may be estimated using Table 4 in MiPNet06.03. For this purpose KCl based media can be described as "seawater" of varying salinity. The original data on sucrose and KCl-media (Reynafarje et al 1985), however, have been critizesed as artefacts and the FM of 0.92 is suggested in the temperature range of 10 °C to 40 °C as for MiR05.
Abbreviation: FM
Reference: MiPNet06.03 POS-calibration-SOP
Keywords
- Bioblast links: Concentration and pressure - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Concentration
- » Volume
- » Activity
- » Concentration
- » Density
- » Mole
- » Molar mass
- Concentration
- Pressure
- Solubility = concentration/pressure
- General
- » Boltzmann constant
- » Energy
- » Force
- » Gas constant
- » Work
- General
- Related keyword lists
- Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- O2k-Procedures
- MiPNet O2k-Procedures
- General
- » Oxygen, dioxygen, O2
- » Oxygen calibration - DatLab
- » Oxygen solubility
- » Oxygen solubility factor
- » Oxygen pressure
- » Concentration
- » Activity
- » Pressure - Pascal
- » Barometric pressure
- » High-resolution respirometry
- » OroboPOS
- » Polarographic oxygen sensor
- » MitoFit Quality Control System
- » Sensitivity
- General
- Other keyword lists
MitoPedia methods: Respirometry
MitoPedia O2k and high-resolution respirometry:
DatLab
MitoPedia topics:
Media for respirometry
Labels:
HRR: Theory