Difference between revisions of "P-L net OXPHOS capacity"
Beno Marija (talk | contribs) |
|||
Line 7: | Line 7: | ||
|mitopedia concept=Respiratory state | |mitopedia concept=Respiratory state | ||
}} | }} | ||
{{MitoPedia | {{MitoPedia topics | ||
|mitopedia topic=EAGLE | |||
}} | |||
[[File:EPL-free and excess.jpg|right|240px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]] | [[File:EPL-free and excess.jpg|right|240px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]] | ||
Revision as of 14:37, 18 November 2016
Description
The free OXPHOS capacity, βP, is the OXPHOS capacity corrected for LEAK respiration, βP = P-L. βP is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS capacity, βP, strictly coupled to phosphorylation, ~P, and nonphosphorylating LEAK respiration, LP, compensating for proton leaks, slip and cation cycling: P = βP+LP. It is frequently assumed that LEAK respiration, L, as measured in the LEAK state, overestimates the LEAK component of respiration, LP, as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in L and LP. However, if the LEAK component increases with enzyme turnover during P, the low enzyme turnover during L may counteract the effect of the higher Ξpmt.
Abbreviation: βP
Reference: Gnaiger 2014 MitoPathways
MitoPedia concepts:
Respiratory state
MitoPedia topics:
EAGLE
Coupling control states for βP
- Reference state, ZX: OXPHOS capacity, P = PΒ΄-ROX
- Background state, YX: LEAK respiration, L = LΒ΄-ROX
- Metabolic control variable, X=ZX-YX: Scope of ADP stimulation, free OXPHOS capacity, βP = P-L
Flux control factor
- Β» Flux control factor, FCF
- Coupling control factor, 1-YX/ZX: P-L coupling control factor, OXPHOS coupling efficiency: jβP = βP/P =(P-L)/P = 1-L/P
Compare
- Free ETS capacity, βE = E-L
- netOXPHOS control ratio, βP/E control ratio: βP/E = (P-L)/E
- ETS coupling efficiency, E-L control factor: jβE = βE/E = (E-L)/E = 1-L/E
- Intact cells: Free ROUTINE activity, βR = R-L