Difference between revisions of "Pichaud 2012 Evolution"
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|title=Pichaud N, Ballard JWO, Tanguay RM, Blier PU (2012) Naturally occurring mitochondrial DNA haplotypes exhibit metabolic differences: insight into functional properties of mitochondria. Evolution [Epub ahead of print]. | |title=Pichaud N, Ballard JWO, Tanguay RM, Blier PU (2012) Naturally occurring mitochondrial DNA haplotypes exhibit metabolic differences: insight into functional properties of mitochondria. Evolution [Epub ahead of print]. | ||
|info=[http://onlinelibrary.wiley.com/doi/10.1111/j.1558-5646.2012.01683.x/abstract?userIsAuthenticated=false&deniedAccessCustomisedMessage= DOI: 10.1111/j.1558-5646.2012.01683.x] | |info=[http://onlinelibrary.wiley.com/doi/10.1111/j.1558-5646.2012.01683.x/abstract?userIsAuthenticated=false&deniedAccessCustomisedMessage= DOI: 10.1111/j.1558-5646.2012.01683.x] | ||
|authors=Pichaud N, Ballard JWO, Tanguay RM, Blier PU | |authors=Pichaud N, Ballard JWO, Tanguay RM, Blier PU | ||
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|abstract=Linking the mitochondrial genotype and the organismal phenotype is of paramount importance in evolution of mitochondria. In this study, we determined the differences in catalytic properties of mitochondria dictated by divergences in the siII and siIII haplogroups of Drosophila simulans using introgressions of siII mtDNA type into the siIII nuclear background. We used a novel in situ method (permeabilized fibers) that allowed us to accurately measure the consumption of oxygen by mitochondria in constructed siII-introgressed flies and in siIII-control flies. Our results showed that the catalytic capacity of the electron transport system is not impaired by introgressions, suggesting that the functional properties of mitochondria are tightly related to the mtDNA haplogroup and not to the nuclear DNA or to the mito-nuclear interactions. This is the first study, to our knowledge, that demonstrates a naturally occurring haplogroup can confer specific functional differences in aspects of mitochondrial metabolism. This study illustrates the importance of mtDNA changes on organelle evolution and highlights the potential bioenergetic and metabolic impacts that divergent mitochondrial haplogroups may have upon a wide variety of species including humans. | |abstract=Linking the mitochondrial genotype and the organismal phenotype is of paramount importance in evolution of mitochondria. In this study, we determined the differences in catalytic properties of mitochondria dictated by divergences in the siII and siIII haplogroups of Drosophila simulans using introgressions of siII mtDNA type into the siIII nuclear background. We used a novel in situ method (permeabilized fibers) that allowed us to accurately measure the consumption of oxygen by mitochondria in constructed siII-introgressed flies and in siIII-control flies. Our results showed that the catalytic capacity of the electron transport system is not impaired by introgressions, suggesting that the functional properties of mitochondria are tightly related to the mtDNA haplogroup and not to the nuclear DNA or to the mito-nuclear interactions. This is the first study, to our knowledge, that demonstrates a naturally occurring haplogroup can confer specific functional differences in aspects of mitochondrial metabolism. This study illustrates the importance of mtDNA changes on organelle evolution and highlights the potential bioenergetic and metabolic impacts that divergent mitochondrial haplogroups may have upon a wide variety of species including humans. | ||
|keywords=Catalytic capacity, electron transport system, haplogroup, introgression, mitochondrial DNA, Drosophila simulans | |keywords=Catalytic capacity, electron transport system, haplogroup, introgression, mitochondrial DNA, Drosophila simulans | ||
|mipnetlab=AU Sydney Ballard JWO, | |mipnetlab=AU Sydney Ballard JWO, CA Rimouski Blier PU, | ||
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Revision as of 14:31, 31 May 2012
| Pichaud N, Ballard JWO, Tanguay RM, Blier PU (2012) Naturally occurring mitochondrial DNA haplotypes exhibit metabolic differences: insight into functional properties of mitochondria. Evolution [Epub ahead of print]. |
» DOI: 10.1111/j.1558-5646.2012.01683.x
Pichaud N, Ballard JWO, Tanguay RM, Blier PU (2012) Evolution
Abstract: Linking the mitochondrial genotype and the organismal phenotype is of paramount importance in evolution of mitochondria. In this study, we determined the differences in catalytic properties of mitochondria dictated by divergences in the siII and siIII haplogroups of Drosophila simulans using introgressions of siII mtDNA type into the siIII nuclear background. We used a novel in situ method (permeabilized fibers) that allowed us to accurately measure the consumption of oxygen by mitochondria in constructed siII-introgressed flies and in siIII-control flies. Our results showed that the catalytic capacity of the electron transport system is not impaired by introgressions, suggesting that the functional properties of mitochondria are tightly related to the mtDNA haplogroup and not to the nuclear DNA or to the mito-nuclear interactions. This is the first study, to our knowledge, that demonstrates a naturally occurring haplogroup can confer specific functional differences in aspects of mitochondrial metabolism. This study illustrates the importance of mtDNA changes on organelle evolution and highlights the potential bioenergetic and metabolic impacts that divergent mitochondrial haplogroups may have upon a wide variety of species including humans. • Keywords: Catalytic capacity, electron transport system, haplogroup, introgression, mitochondrial DNA, Drosophila simulans
• O2k-Network Lab: AU Sydney Ballard JWO, CA Rimouski Blier PU
Labels:
Organism: Other Non-Mammal"Other Non-Mammal" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
