Difference between revisions of "Podrabsky 2000 Am J Physiol Regul Integr Comp Physiol"
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|year=2000 | |year=2000 | ||
|journal=Am J Physiol Regul Integr Comp Physiol | |journal=Am J Physiol Regul Integr Comp Physiol | ||
|abstract=A previous phylogenetic | |abstract=A previous phylogenetic analysis among 15 taxa of the teleost fish ''Fundulus'' suggested that there should be thermal-adaptive differences in heart metabolism among populations. To test this hypothesis, the rate of oxygen consumption and the activities of all 11 glycolytic enzymes were measured in isolated heart ventricle from two populations of ''Fundulus heteroclitus''. Heart ventricular metabolism is greater in a northern population versus a southern population of these fish. Analysis of the amount of glycolytic enzymes indicates that 87% of the variation in cardiac metabolism within and between populations is explained by the variation in three enzymes (pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase, and lactate dehydrogenase). These enzymes are the same three enzymes that were predicted to be important based on previously determined phylogenetic patterns of expression. Our data indicate that near-equilibrium enzymes, as well as classically defined rate-limiting enzymes, can also influence metabolism. | ||
analysis among 15 taxa of the teleost fish ''Fundulus'' suggested | |||
that there should be thermal-adaptive differences in | |||
heart metabolism among populations. To test this hypothesis, | |||
the rate of oxygen consumption and the activities of all 11 | |||
glycolytic enzymes were measured in isolated heart ventricle | |||
from two populations of ''Fundulus heteroclitus''. Heart ventricular | |||
metabolism is greater in a northern population versus a | |||
southern population of these fish. Analysis of the amount of | |||
glycolytic enzymes indicates that 87% of the variation in | |||
cardiac metabolism within and between populations is explained | |||
by the variation in three enzymes (pyruvate kinase, | |||
glyceraldehyde-3-phosphate dehydrogenase, and lactate dehydrogenase). | |||
These enzymes are the same three enzymes | |||
that were predicted to be important based on previously | |||
determined phylogenetic patterns of expression. Our data | |||
indicate that near-equilibrium enzymes, as well as classically | |||
defined rate-limiting enzymes, can also influence metabolism. | |||
|keywords=Evolution, Cardiac adaptation | |keywords=Evolution, Cardiac adaptation | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Fish | |organism=Fish | ||
|tissues=Cardiac | |tissues=Cardiac muscle | ||
|additional=DatLab | |additional=DatLab | ||
}} | }} | ||
Revision as of 07:17, 5 April 2012
| Podrabsky JE, Javillonar C, Hand SC, Crawford DL (2000) Intraspecific variation in aerobic metabolism and glycolytic enzyme expression in heart ventricles. Am J Physiol Regul Integr Comp Physiol 279: R2344-R2348. |
Podrabsky JE, Javillonar C, Hand SC, Crawford DL (2000) Am J Physiol Regul Integr Comp Physiol
Abstract: A previous phylogenetic analysis among 15 taxa of the teleost fish Fundulus suggested that there should be thermal-adaptive differences in heart metabolism among populations. To test this hypothesis, the rate of oxygen consumption and the activities of all 11 glycolytic enzymes were measured in isolated heart ventricle from two populations of Fundulus heteroclitus. Heart ventricular metabolism is greater in a northern population versus a southern population of these fish. Analysis of the amount of glycolytic enzymes indicates that 87% of the variation in cardiac metabolism within and between populations is explained by the variation in three enzymes (pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase, and lactate dehydrogenase). These enzymes are the same three enzymes that were predicted to be important based on previously determined phylogenetic patterns of expression. Our data indicate that near-equilibrium enzymes, as well as classically defined rate-limiting enzymes, can also influence metabolism. • Keywords: Evolution, Cardiac adaptation
Labels:
Organism: Fish"Fish" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Tissue;cell: Cardiac muscle"Cardiac muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
DatLab
