Difference between revisions of "Racker 1974 J Biol Chem"
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{{Publication | {{Publication | ||
|title=Racker E, Stoeckenius W (1974) Reconstitution of purple membrane vesicles catalyzing light-driven proton uptake and adenosine triphosphate formation. J Biol Chem 249: 662- | |title=Racker E, Stoeckenius W (1974) Reconstitution of purple membrane vesicles catalyzing light-driven proton uptake and adenosine triphosphate formation. J Biol Chem 249:662-3. | ||
|info=[http://www.jbc.org/content/249/2/662.full.pdf+html PMID: 4272126 Open Access] | |info=[http://www.jbc.org/content/249/2/662.full.pdf+html PMID: 4272126 Open Access] | ||
|authors=Racker E, Stoeckenius W | |authors=Racker E, Stoeckenius W | ||
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{{Labeling | {{Labeling | ||
|taxonomic group=Eubacteria | |taxonomic group=Eubacteria | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|enzymes=Complex V; ATP | |enzymes=Complex V;ATP synthase | ||
|topics=ADP, Coupling efficiency;uncoupling | |topics=ADP, Coupling efficiency;uncoupling | ||
|additional=Made history | |additional=Made history | ||
}} | }} | ||
Revision as of 16:56, 11 February 2015
| Racker E, Stoeckenius W (1974) Reconstitution of purple membrane vesicles catalyzing light-driven proton uptake and adenosine triphosphate formation. J Biol Chem 249:662-3. |
Racker E, Stoeckenius W (1974) J Biol Chem
Abstract: The purple membrane from Halobacterium halobium was incorporated into phospholipid vesicles. On illumination (2 X 106 ergs per cm2 per s) the reconstituted vesicles took up protons (50 to 200 ng of ions H+ per mg of purple protein) which were released in the dark. Addition of valinomycin accelerated both the rate of uptake in the light and the release of protons in the dark. Uncouplers of oxidative phosphorylation abolished the uptake of protons. Inclusion of the mitochondrial oligomycin-sensitive ATPase during reconstitution yielded vesicles which catalyzed light-dependent phosphorylation. These reconstituted vesicles represent a simple model system for a biological proton pump capable of generating ATP from ADP and Pi. • Keywords: Halobacterium halobium, purple membrane vesicles, proton uptake, ATP, light
Labels:
Preparation: Isolated mitochondria
Enzyme: Complex V;ATP synthase
Regulation: ADP, Coupling efficiency;uncoupling
Made history
