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Difference between revisions of "Respiratory state"

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{{MitoPedia
{{MitoPedia
|abbr=n.a.
|description='''Respiratory states''' of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the [[coupling control state]] and the [[substrate control state]].
|description='''Respiratory states''' of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the [[coupling control state]] and the [[substrate control state]].
|info=[[Gnaiger 2014 MitoPathways]]
|info=[[Gnaiger 2014 MitoPathways]]
}}
}}
{{MitoPedia concepts}}
{{MitoPedia methods
{{MitoPedia methods
|mitopedia method=Respirometry, Spectrophotometry
|mitopedia method=Respirometry, Spectrophotometry
}}
}}
{{MitoPedia SUIT}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Respiratory state
|mitopedia topic=Respiratory state

Revision as of 22:22, 27 January 2016


high-resolution terminology - matching measurements at high-resolution


Respiratory state

Description

Respiratory states of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling control state and the substrate control state.


Reference: Gnaiger 2014 MitoPathways



MitoPedia methods: Respirometry, Spectrophotometry 

Template:MitoPedia SUIT

MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 

OXPHOS
ROUTINE
ETS
LEAK
ROX

Coupling control states

Coupling states and CCR of mitochondrial preparations:

Coupling states of intact cells:

Substrate control states

Substrate control states, SCR, are defined by substrate type (at saturating concentration) as pathway control states:

Control by substrate concentration: Kinetic control states:

  • Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
  • Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.

Classical respiratory states

Chance and Williams (1955):

Derived respiratory states:

Thermodynamics of irreversible processes:

MitoPedia: Respiratory states