Difference between revisions of "Respiratory state"
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|mitopedia concept=MitoFit Quality Control System | |mitopedia concept=Respiratory state, MitoFit Quality Control System | ||
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|mitopedia method=Respirometry, Spectrophotometry | |mitopedia method=Respirometry, Spectrophotometry | ||
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[[Image:P.jpg|right|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|right|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|right|link=ETS capacity|ETS]] [[Image:L.jpg|right|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|right|link=Residual oxygen consumption|ROX]] | [[Image:P.jpg|right|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|right|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|right|link=ETS capacity|ETS]] [[Image:L.jpg|right|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|right|link=Residual oxygen consumption|ROX]] | ||
== Coupling control states == | == Coupling control states == | ||
Revision as of 13:36, 8 February 2016
- high-resolution terminology - matching measurements at high-resolution
Respiratory state
Description
Respiratory states of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling control state and the substrate control state.
Reference: Gnaiger 2014 MitoPathways
MitoPedia concepts:
Respiratory state, "MitoFit Quality Control System" is not in the list (MiP concept, Respiratory state, Respiratory control ratio, SUIT concept, SUIT protocol, SUIT A, SUIT B, SUIT C, SUIT state, Recommended, ...) of allowed values for the "MitoPedia concept" property.
MitoFit Quality Control System"MitoFit Quality Control System" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
MitoPedia methods:
Respirometry,
Spectrophotometry
Coupling control states
Coupling states and CCR of mitochondrial preparations:
Coupling states of intact cells:
Substrate control states
Substrate control states, SCR, are defined by substrate type (at saturating concentration) as pathway control states:
- Intact cells: endogenous, exogenous substrate control
- Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with fatty acid oxidation, alpha-glycerophosphate, (Complex I-linked substrate state, Complex II-linked substrate state, etc.), or substrate combinations applied for reconstitution of TCA cycle function (e.g. Complex I&II-linked substrate state, etc.).
Control by substrate concentration: Kinetic control states:
- Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
- Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.
Classical respiratory states
Chance and Williams (1955):
Derived respiratory states:
Thermodynamics of irreversible processes:
MitoPedia: Respiratory states
- See the complete MitoPedia: Respiratory states.
