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Difference between revisions of "Respiratory state"

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== Coupling control states ==
== Coupling control states ==


Coupling states and ''[[CCR]]'' of mitochondrial preparations:
::: Coupling states and ''[[CCR]]'' of mitochondrial preparations:


*[[OXPHOS]], [[LEAK]], [[ETS]] (''P, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[P/E]]'', ''[[L/P]]''
::::* [[OXPHOS]], [[LEAK]], [[ETS]] (''P, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[P/E]]'', ''[[L/P]]''


Coupling states of intact cells:
::: Coupling states of intact cells:


*[[ROUTINE]], [[LEAK]], [[ETS]] (''R, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[R/E]]'', ''[[L/R]]''
::::* [[ROUTINE]], [[LEAK]], [[ETS]] (''R, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[R/E]]'', ''[[L/R]]''


== Substrate control states ==
== Substrate control states ==


Substrate control states, ''[[SCR]]'', are defined by substrate type (at saturating concentration) as '''pathway control states''':
:::: Substrate control states, ''[[SCR]]'', are defined by substrate type (at saturating concentration) as '''pathway control states''':


*Intact cells: [[endogenous]], [[exogenous]] substrate control
::::* Intact cells: [[endogenous]], [[exogenous]] substrate control
*Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with [[fatty acid oxidation]], alpha-[[glycerophosphate]], ([[Complex I-linked substrate state]], [[Complex II-linked substrate state]], etc.), or substrate combinations applied for reconstitution of [[TCA cycle]] function (e.g. [[Complex I&II-linked substrate state]], etc.).
::::* Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with [[fatty acid oxidation]], alpha-[[glycerophosphate]], ([[Complex I-linked substrate state]], [[Complex II-linked substrate state]], etc.), or substrate combinations applied for reconstitution of [[TCA cycle]] function (e.g. [[Complex I&II-linked substrate state]], etc.).


Control by substrate concentration: '''Kinetic control states''':
::: Control by substrate concentration: '''Kinetic control states''':


*Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O<sub>2</sub>; cytochrome ''c'') are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as ''J''<sub>max</sub>, ''K''<sub>m</sub>', ''c''<sub>50</sub>, or ''p''<sub>50</sub>.
::::* Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O<sub>2</sub>; cytochrome ''c'') are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as ''J''<sub>max</sub>, ''K''<sub>m</sub>', ''c''<sub>50</sub>, or ''p''<sub>50</sub>.
*Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the ''K''<sub>I</sub>'.
::::* Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the ''K''<sub>I</sub>'.


== Classical respiratory states ==
== Classical respiratory states ==


Chance and Williams (1955):
::: Chance and Williams (1955):


*[[State 1]], [[State 2]], [[State 3]], [[State 4]], [[State 5]]. ''CCR'': [[RCR]]
::::* [[State 1]], [[State 2]], [[State 3]], [[State 4]], [[State 5]]. ''CCR'': [[RCR]]


Derived respiratory states:
::: Derived respiratory states:


*[[State 3u]], [[State 4o]]. ''CCR'': [[UCR]]
::::* [[State 3u]], [[State 4o]]. ''CCR'': [[UCR]]


Thermodynamics of irreversible processes:
::: Thermodynamics of irreversible processes:
Β 
::::* [[Static head]], [[Level flow]]


*[[Static head]], [[Level flow]]


== MitoPedia: Respiratory states ==
== MitoPedia: Respiratory states ==


* See the complete '''[[MitoPedia: Respiratory states]]'''.
::::* See the complete '''[[MitoPedia: Respiratory states]]'''
::::* [[MitoPedia: SUIT]]

Revision as of 08:17, 15 May 2016


high-resolution terminology - matching measurements at high-resolution


Respiratory state

Description

Respiratory states of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling control state and the substrate control state.


Reference: Gnaiger 2014 MitoPathways


MitoPedia concepts: MiP concept, Respiratory state, SUIT concept 


MitoPedia methods: Respirometry, Spectrophotometry 




OXPHOS
ROUTINE
ETS
LEAK
ROX

Coupling control states

Coupling states and CCR of mitochondrial preparations:
Coupling states of intact cells:

Substrate control states

Substrate control states, SCR, are defined by substrate type (at saturating concentration) as pathway control states:
Control by substrate concentration: Kinetic control states:
  • Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
  • Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.

Classical respiratory states

Chance and Williams (1955):
Derived respiratory states:
Thermodynamics of irreversible processes:


MitoPedia: Respiratory states