Difference between revisions of "Respiratory state"
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{{MitoPedia | {{MitoPedia | ||
|description= | |description='''Respiratory states''' of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. The Bioblast Wiki [[List:_Respiratory_states|see ''List of respiratory states'']] aims at providing detailed explanations of terms, improving definitions, and clarification of underlying concepts. | ||
|info=[[MiPNet12.15_RespiratoryStates]]; [[List:_Respiratory_states|List of respiratory states]] | |info=[[MiPNet12.15_RespiratoryStates]]; [[List:_Respiratory_states|List of respiratory states]] | ||
}} | }} | ||
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|instruments=Theory | |instruments=Theory | ||
}} | }} | ||
== | ==Coupling control states== | ||
*[[ | |||
===Coupling states of mitochondrial preparations=== | |||
*[[OXPHOS]], [[LEAK]], [[ETS]] (''P, L, E'') - corrected for [[ROX]]. | |||
===Coupling states of intact cells=== | ===Coupling states of intact cells=== | ||
*[[ROUTINE]], [[LEAK]], [[ETS]] - corrected for [[ROX]]. | *[[ROUTINE]], [[LEAK]], [[ETS]] (''R, L, E'') - corrected for [[ROX]]. | ||
==Substrate control states== | ==Substrate control states== | ||
===Control by substrate type: Pathway control states=== | |||
*Intact cells: [[endogenous]], [[exogenous]] substrate control | *Intact cells: [[endogenous]], [[exogenous]] substrate control | ||
*Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with [[fatty acid]]s and alpha-[[glycerophosphate]] ([[CI respiration]], [[CII respiration]], etc.), or substrate combinations applied for reconstitution of [[TCA cycle]] function ([[CI+II respiration]], etc.). | *Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with [[fatty acid]]s and alpha-[[glycerophosphate]] ([[CI respiration]], [[CII respiration]], etc.), or substrate combinations applied for reconstitution of [[TCA cycle]] function ([[CI+II respiration]], etc.). | ||
===Kinetic control states=== | ===Control by substrate concentration: Kinetic control states=== | ||
*Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O<sub>2</sub>; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as | *Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O<sub>2</sub>; cytochrome ''c'') are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as ''J''<sub>max</sub>, ''K''<sub>m</sub>', ''c''<sub>50</sub>, or ''p''<sub>50</sub>. | ||
*Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the ''K''<sub>I</sub>'. | *Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the ''K''<sub>I</sub>'. | ||
==Classical respiratory states of isolated mitochondria | ==Classical respiratory states of isolated mitochondria== | ||
===Chance and Williams (1955)=== | |||
*[[State 1]], [[State 2]], [[State 3]], [[State 4]], [[State 5]] | *[[State 1]], [[State 2]], [[State 3]], [[State 4]], [[State 5]] | ||
===Derived respiratory states=== | ===Derived respiratory states=== | ||
*[[State 3u]], [[State 4o]] | *[[State 3u]], [[State 4o]] | ||
===Thermodynamics of irreversible processes=== | |||
*[[Static head]], [[Level flow]] | |||
Revision as of 13:30, 27 February 2011
- high-resolution terminology - matching measurements at high-resolution
Respiratory state
Description
Respiratory states of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. The Bioblast Wiki see List of respiratory states aims at providing detailed explanations of terms, improving definitions, and clarification of underlying concepts.
Reference: MiPNet12.15_RespiratoryStates; List of respiratory states
Labels:
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiratory state"Respiratory state" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Redox State"Redox State" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Theory
Coupling control states
Coupling states of mitochondrial preparations
Coupling states of intact cells
Substrate control states
Control by substrate type: Pathway control states
- Intact cells: endogenous, exogenous substrate control
- Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with fatty acids and alpha-glycerophosphate (CI respiration, CII respiration, etc.), or substrate combinations applied for reconstitution of TCA cycle function (CI+II respiration, etc.).
Control by substrate concentration: Kinetic control states
- Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
- Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.
