Respiratory state

high-resolution terminology - matching measurements at high-resolution

Respiratory state

Description

Respiratory states of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. The Bioblast Wiki aims at providing detailed explanations of terms, improving definitions, and clarification of underlying concepts - see List of respiratory states.

Reference: MiPNet12.15_RespiratoryStates; List of respiratory states

Labels:

Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 

Regulation: Respiratory state"Respiratory state" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Redox State"Redox State" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 

HRR: Theory 

Coupling control states

Coupling states and CCR of mitochondrial preparations:

• OXPHOS, LEAK, ETS (P, L, E) - corrected for ROX. CCR: L/E, P/E, L/P

Coupling states of intact cells:

• ROUTINE, LEAK, ETS (R, L, E) - corrected for ROX. CCR: L/E, R/E, L/R

Substrate control states

Control by substrate type: Pathway control states:

• Intact cells: endogenous, exogenous substrate control
• Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with fatty acids and alpha-glycerophosphate (CI respiration, CII respiration, etc.), or substrate combinations applied for reconstitution of TCA cycle function (CI+II respiration, etc.).

Control by substrate concentration: Kinetic control states:

• Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O₂; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as J_max, K_m', c₅₀, or p₅₀.
• Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the K_I'.

Classical respiratory states

Chance and Williams (1955):

• State 1, State 2, State 3, State 4, State 5. CCR: RCR

Derived respiratory states:

• State 3u, State 4o. CCR: UCR

Thermodynamics of irreversible processes:

• Static head, Level flow