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Difference between revisions of "Respiratory state"

From Bioblast
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|mitopedia topic=EAGLE
|mitopedia topic=EAGLE
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Β  Communicated by [[Gnaiger E]] 2010-10-21, edited 2016-08-26. Edited by [[Doerrier Carolina]] 2020-04-23.
Β  Communicated by [[Gnaiger E]] 2010-10-21, edited 2016-08-26. Edited by [[Doerrier Carolina|Doerrier C]] 2020-04-23.
[[Image:P.jpg|right|link=OXPHOS-capacity|OXPHOS]] [[Image:R.jpg|right|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|right|link=ET-capacity|ETS]] [[Image:L.jpg|right|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|right|link=Residual oxygen consumption|ROX]]
[[Image:P.jpg|right|link=OXPHOS-capacity|OXPHOS]] [[Image:R.jpg|right|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|right|link=ET-capacity|ETS]] [[Image:L.jpg|right|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|right|link=Residual oxygen consumption|ROX]]
== Coupling control states ==
== Coupling control states ==

Revision as of 12:01, 23 April 2020


high-resolution terminology - matching measurements at high-resolution


Respiratory state

Description

Respiratory states of mitochondrial preparations and living cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling control state and the electron transfer-pathway state.


Reference: Gnaiger 2014 MitoPathways


MitoPedia concepts: MiP concept, Respiratory state, SUIT concept, Recommended 


MitoPedia methods: Respirometry, Spectrophotometry 


MitoPedia topics: EAGLE 

Communicated by Gnaiger E 2010-10-21, edited 2016-08-26. Edited by Doerrier C 2020-04-23.
OXPHOS
ROUTINE
ETS
LEAK
ROX

Coupling control states

Coupling control states and CCR of mitochondrial preparations:
Coupling control states of living cells:

Electron transfer-pathway state

Electron transfer-pathway state, are defined by substrate type (at saturating concentration):
Control by substrate concentration: Kinetic control states:
  • Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
  • Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.

Classical respiratory states

Chance and Williams (1955):
Derived respiratory states:
Thermodynamics of irreversible processes:


MitoPedia: Respiratory states

Β» See the complete MitoPedia: Respiratory states
Β» MitoPedia: SUIT