Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Respiratory state"

From Bioblast
Line 25: Line 25:


*Intact cells: [[endogenous]], [[exogenous]] substrate control
*Intact cells: [[endogenous]], [[exogenous]] substrate control
*Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with [[fatty acid]]s and alpha-[[glycerophosphate]] ([[CI respiration]], [[CII respiration]], etc.), or substrate combinations applied for reconstitution of [[TCA cycle]] function ([[CI+II respiration]], etc.).
*Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with [[fatty acid]]s and alpha-[[glycerophosphate]] ([[CI-linked respiration]], [[CII-linked respiration]], etc.), or substrate combinations applied for reconstitution of [[TCA cycle]] function ([[CI&II-linked respiration]], etc.).


Control by substrate concentration: '''Kinetic control states''':
Control by substrate concentration: '''Kinetic control states''':

Revision as of 23:37, 3 September 2014


high-resolution terminology - matching measurements at high-resolution


Respiratory state

Description

Respiratory states of mitochondrial preparations and intact cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling control state and the substrate control state.

Abbreviation: n.a.

Reference: Gnaiger 2014 MitoPathways


MitoPedia methods: Respirometry, Spectrophotometry 


MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 

Coupling control states

Coupling states and CCR of mitochondrial preparations:

Coupling states of intact cells:

Substrate control states

Substrate control states, SCR, are defined by substrate type (at saturating concentration) as pathway control states:

Control by substrate concentration: Kinetic control states:

  • Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
  • Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.

Classical respiratory states

Chance and Williams (1955):

Derived respiratory states:

Thermodynamics of irreversible processes:

Glossary: Respiratory states