Difference between revisions of "Rhodamine 123"
From Bioblast
(Created page with "{{MitoPedia |abbr=Rhodamine 123 |description= |info= }} {{Keywords Force and membrane potential}} __TOC__") Β |
|||
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=Rh123 | ||
|description= | |description= Rhodamine 123 is an [[extrinsic fluorophores|extrinsic fluorophore]] and can be used as a probe to determine changes in [[Mitochondrial_membrane_potential|mitochondrial membrane potential]]. Rh123 is a lipophilic cation that is accumulated by mitochondria in proportion to Ξ''Ο''<sub>mt</sub>. Using ethanol as the solvent, the excitation maximum is511 nm and the emission maximum is 534 nm.The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively (Sigma-Aldrich). | ||
|info= | |info= | ||
}} | }} | ||
| Line 7: | Line 7: | ||
__TOC__ | __TOC__ | ||
== Application in [[HRFR]] == | |||
== Signal and output == | |||
== Rh123 with the [[O2k-FluoRespirometer]]: exploratory experiments == | |||
{{MitoPedia methods | |||
|mitopedia method=Fluorometry | |||
}} | |||
{{Labeling | |||
|area=Respiration, Instruments;methods | |||
|instruments=Oxygraph-2k, O2k-Fluorometer, O2k-Protocol | |||
|additional=O2k-MultiSensor | |||
}} | |||
Revision as of 12:47, 13 March 2019
- high-resolution terminology - matching measurements at high-resolution
Rhodamine 123
Description
Rhodamine 123 is an extrinsic fluorophore and can be used as a probe to determine changes in mitochondrial membrane potential. Rh123 is a lipophilic cation that is accumulated by mitochondria in proportion to ΞΟmt. Using ethanol as the solvent, the excitation maximum is511 nm and the emission maximum is 534 nm.The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively (Sigma-Aldrich).
Abbreviation: Rh123
Template:Keywords Force and membrane potential
Application in HRFR
Signal and output
Rh123 with the O2k-FluoRespirometer: exploratory experiments
MitoPedia methods: Fluorometry
Labels: MiParea: Respiration, Instruments;methods
HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-MultiSensor
