Rhodamine 123

high-resolution terminology - matching measurements at high-resolution

Rhodamine 123

Description

Rhodamine 123 is an extrinsic fluorophore and can be used as a probe to determine changes in mitochondrial membrane potential. Rh123 is a lipophilic cation that is accumulated by mitochondria in proportion to Δψ_mt. Using ethanol as the solvent, the excitation maximum is511 nm and the emission maximum is 534 nm.The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively (Sigma-Aldrich).

Abbreviation: Rh123

Rh123 with the O2k-FluoRespirometer: exploratory experiments

MitoPedia methods: Fluorometry 

Labels: MiParea: Respiration, Instruments;methods 

HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol 

O2k-MultiSensor