Rosenfeld 2010 Abstract IOC60

Rosenfeld E (2010) Abstract IOC60

Link: IOC60

Rosenfeld E (2010)

Event: IOC60

There are many living organisms that produce redox-active toxins targeting the respiratory chain. Producers and targets are either eukaryotic or prokaryotic cells. For instance, the opportunistic pathogen bacterium Pseudomonas aeruginosa is known to produce the complex IV inhibitor HCN and a blue phenazine pigment, pyocyanin (PCN), which harbors many potential redox properties. During infection (e.g. lung infection in cystic fibrosis patients), the bacterium can by-pass the effect of HCN by expressing a cyanide-insensitive quinol oxidase. It can also shift its metabolism from micro-aerobic to anaerobic respiration. The modes of action of PCN on its producer (P. aeruginosa) and on its prokaryotic and eukaryotic targets remain unclear. For example, it is unknown whether the redox recycling of PCN absolutely requires respiratory chain activity to induce ROS overproduction. A methodology based on HRR (high-Resolution Respirometry) is currently being designed to precise the cellular targets of PCN and other related compounds in prokaryotic and eukaryotic cells, under aerobic and micro-aerobic conditions. The yeast Saccharomyces cerevisiae is being used to determine the effect of PCN under different levels of respiratory competency. This implies the use of (i) several oxygenation conditions for growth of wild-type and mutant strains, (ii) respiratory inhibitors, and (iii) a panel of inhibitors of NADPH-dependent non-respiratory oxygen consumption pathways.

• Keywords: Pseudomonas aeruginosa, pyocyanin, respiratory chain, Saccharomyces cerevisiae, non-respiratory oxygen consumption pathways.

Labels:

Stress:Hypoxia, RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.  Organism: Yeast; Fungi"Yeast; Fungi" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property., Bacteria"Bacteria" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property. 

Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Oxidase; Biochemical Oxidation"Oxidase; Biochemical Oxidation" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 

Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Aerobic and Anaerobic Metabolism"Aerobic and Anaerobic Metabolism" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Redox State"Redox State" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 

HRR: Oxygraph-2k, Spectrophotometry; Spectrofluorimetry"Spectrophotometry; Spectrofluorimetry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., DatLab Software; Separate Application"DatLab Software; Separate Application" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.  Event: IOC60"IOC60" is not in the list (A1, A2, A3, A4, B1, B2, B3, B4, C1, C2, ...) of allowed values for the "Event" property.