SUIT-003 O2 ce D012: Difference between revisions
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::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceN(P) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceN(P) | ||
::: '''[[SUIT protocol pattern]]:''' complex cell [[coupling control protocol]] ce1;ce2P;ce3Omy;ce4U | ::: '''[[SUIT protocol pattern]]:''' complex cell [[coupling control protocol]] ce1;ce2P;ce3Omy;ce4U | ||
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{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT protocol, SUIT A, Find | |mitopedia concept=SUIT protocol, SUIT A, Find | ||
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{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
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== References == | |||
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Revision as of 16:52, 18 January 2019
Description
Abbreviation: ceN(P)
Reference: A - SUIT-003
SUIT number: D012_ce1;ce2P;ce3Omy;ce4U;ce5Rot;ce6Ama
O2k-Application: O2
- SUIT-category: ceN(P)
- SUIT protocol pattern: complex cell coupling control protocol ce1;ce2P;ce3Omy;ce4U
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state. | ||
ce1P | ROUTINE | ce1;ce1P. Pyruvate, ROUTINE, R
ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state. | ||
ce2Omy | L | ce1;ce1P;ce2Omy
Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates).
| ||
ce3U | E | ce1;ce1P;ce2Omy;ce3U
Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. Noncoupled electron transfer state, ET state, with ET capacity E. | ||
ce4Rot | ROX | ce1;ce1P;ce2Omy;ce3U;ce4Rot
| ||
ce5Ama | ROX | ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5Ama
|
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry