Difference between revisions of "SUIT-006 O2 mt D022"
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|SUIT number=D022_1SRot;2D;2c;3U;4Ama | |SUIT number=D022_1SRot;2D;2c;3U;4Ama | ||
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::: [[MitoPedia: SUIT]] | ::: '''[[MitoPedia: SUIT]]''' | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' S | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' S | ||
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] 1SRot;2D;2c;3U;4Ama | ::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] 1SRot;2D;2c;3U;4Ama |
Revision as of 13:22, 6 March 2019
Description
Abbreviation: S
Reference: A SUIT-006
SUIT number: D022_1SRot;2D;2c;3U;4Ama
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: S
- SUIT protocol pattern: linear coupling control protocol 1SRot;2D;2c;3U;4Ama
SUIT-006 O2 mt D022 is a coupling control protocol for for mitochondrial preparations (tissue homogenate and isolated mitochondria). Different coupling control states are assessed (L(n) - P - E) at succinate pathway control state.
Communicated by Cardoso LH, Komlodi T, Huete-Ortega M and Doerrier C (last update 2019-03-05)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1SRot | SL | S | CII | 1SRot
|
2D | SP | S | CII | 1SRot;2D
|
2c | ScP | S | CII | 1SRot;2D;2c
|
(3Omy) | ScL(Omy) | 1SRot;2D;2c;(3Omy)
| ||
4U | SE | S | CII | 1SRot;2D;2c;4U
|
5Ama | ROX | 1SRot;2D;2c;4U;5Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol is indicated when the aim is to analyse coupling control in mtprep (see MitoPedia: Sample preparations) at Succinate pathway control state.
- + The combination of succinate with rotenone, that inhibits complex I, provides the best coupling control protocol specific for S-pathway, avoiding reverse electron transfer (RET).
- + Reasonable duration of the experiment.
- + This protocol can be extended with the Complex IV module.
- - This protocol does not include internal ET-pathway control steps.
- - Careful washing is required after the experiment to avoid carry-over of uncoupler and inhibitors.
Compare SUIT protocols
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Krumschnabel 2014 Methods Enzymol | 2014 | Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. https://doi.org/10.1016/B978-0-12-416618-9.00009-1 | Mouse | Nervous system |
MitoPedia concepts:
SUIT protocol,
SUIT A,
Find
MitoPedia methods:
Respirometry