From Bioblast
- high-resolution terminology - matching measurements at high-resolution
SUIT-008 O2 pfi D014
Description
Abbreviation: NS(PGM)
Reference: A: SUIT 8 is the SUIT protocol used in the MitoEAGLE project (WG2) for testing the skill preparing permeabilized fibers Lemieux 2017 Sci Rep
SUIT number: 8
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: NS(PGM)
- SUIT protocol pattern: diametral.
References
| Year | Reference | Organism | Tissue;cell | |
|---|---|---|---|---|
| Lemieux 2017 Sci Rep | 2017 | Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8 | Mouse | Heart |
1PM;2D;2c;3G;4S;5U;6Rot-
1PM;2D;2c;3G;4S;5U;6Rot;7Ama
| Step | Respiratory state | Pathway control | Entry through electron transfer Complex | Comments |
|---|---|---|---|---|
| 1PM | PM(L) | N | CI | |
| 2D | PM(P) | N | CI | |
| D(c) | PM(c) | N | CI | |
| 3G | PGM(P) | N | CI | |
| 4S | PGMS(P) | NS | CI&II | |
| 5U | PGMS(E) | NS | CI&II | |
| 6Rot | S(E) | S | CII | |
| 7Ama | ROX |
1PM;2D;3G;3c;4S;5U;6Rot-
1PM;2D;3G;3c;4S;5U;6Rot;7Ama
| Step | Respiratory state | Pathway control | Entry through electron transfer Complex | Comments |
|---|---|---|---|---|
| 1PM | PM(L) | N | CI | |
| 2D | PM(P) | N | CI | |
| 3G | PGM(P) | N | CI | |
| G(c) | PGM(c) | N | CI | Limitation: Cytochrome c should be added after step 2D, such that all OXPHOS states can be compared in the presence of cytochrome c. See 1PM;2D;2c;3G;4S;5U;6Rot-. |
| 4S | PGMS(P) | NS | CI&II | |
| 5U | PGMS(E) | NS | CI&II | |
| 6Rot | S(E) | S | CII | |
| 7Ama | ROX |
SUIT O2 DLP for SUIT 8 (DatLab 7.1)
- DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols MitoPedia: SUIT. Instrumental DL-protocols are displayed in the DL-Protocol library.
Download instructions.
To start the download click on theicon. Depending on your browser you will encounter different download scenarios.
(i) A window opens to select whether to "open file with" or to "save file". Please always choose the option "save file"; opening the file directly will not work due to safety reasons.
(ii) When you click the download icon, some browsers will display the text/content of the .DLP file instead of downloading it. In this case go back to this download page and right-click the download icon and choose "save linked file as" from the context-menu. A window will open to save the file. In some cases the suffix of the file might be wrong (e.g. ".HTML") - you must correct the suffix of the file to ".DLP".
| DL-Protocol | Description | Download - Protocol .DLP | Download - Analysis .XLSX |
|---|---|---|---|
| SUIT 8_pfi | SUIT protocol used in the MitoEAGLE project (WG2) for testing the skill preparing permeabilized fibers (pfi) | work in progress | work in progress |
