SUIT-009 O2 ce-pce D016: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 3: | Line 3: | ||
|description=[[File:1S;2D;3P;4Rot-.png|300px|SUIT-009]] | |description=[[File:1S;2D;3P;4Rot-.png|300px|SUIT-009]] | ||
|info='''A''': '''[[SUIT-009]]''' short protocol for mt-respirationΒ in [[permeabilized cells |pce]]. | |info='''A''': '''[[SUIT-009]]''' short protocol for mt-respirationΒ in [[permeabilized cells |pce]]. | ||
|SUIT number=D016_ce1;1Dig;1S;2D;3P;4Rot;5Ama | |||
|O2k-Application=O2 | |||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(P) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(P) |
Revision as of 16:20, 21 January 2019
Description
Abbreviation: NS(P)
Reference: A: SUIT-009 short protocol for mt-respiration in pce.
SUIT number: D016_ce1;1Dig;1S;2D;3P;4Rot;5Ama
- SUIT-category: NS(P)
- SUIT protocol pattern: orthogonal 1S;2D;3P;4Rot;5Ama
SUIT protocols
References
Strengths and limitations
- For H2O2-Application, choose SUIT-009 AmR pce D019; for O2 and H2O2 measurements (AmR), choose: SUIT-009 AmR pce D019.
- Cytochrome c test can be performed in the S-OXPHOS state.
- Reasonable duration of the experiment.
- - In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry