Difference between revisions of "SUIT-010"
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|abbr=ceS,S | |abbr=ceS,S | ||
|description=[[File:ce1;ce2Rot;ce3S;ce4D;1Dig;1c.png|400px|Respirometric test of optimum digitonin concentration ]] | |description=[[File:ce1;ce2Rot;ce3S;ce4D;1Dig;1c.png|400px|Respirometric test of optimum digitonin concentration ]] | ||
|info='''A | |info='''A''' Optimum [[digitonin]] concentration for [[permeabilized cells |pce]] [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-010.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:SUIT-010.pdf Versions] | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceS,S | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceS,S |
Revision as of 18:10, 21 January 2019
Description
Abbreviation: ceS,S
Reference: A Optimum digitonin concentration for pce »Versions
- SUIT-category: ceS,S
- SUIT protocol pattern: ce1;ce2Rot;ce3S;ce4D;1Dig;1c
DLP applications
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Doerrier 2018 Methods Mol Biol | 2018 | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 | Human Mouse Rat Saccharomyces cerevisiae | Heart Skeletal muscle Endothelial;epithelial;mesothelial cell Blood cells HEK Platelet |
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2Rot | ROX | ce1;ce2Rot
| ||
ce3S | SL(n) | S | CII | ce1;ce2Rot;ce3S
|
ce4D | SP | S | CII | ce1;ce2Rot;ce3S;ce4D
|
1Dig | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig
|
1c | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig;1c
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- Digitonin is a natural product and thus the effective concentration has to be determined by titrations for every batch. The optimum effective digitonin concentrations for complete plasma membrane permeabilization can be determined with this protocol.
Compare SUIT protocols
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry