Difference between revisions of "SUIT protocol library"
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'''''This page is in preparation''''' (intern wiki: | '''''This page is in preparation''''' (intern wiki: SUIT protocol library intern) | ||
<big><big>'''[[Substrate-uncoupler-inhibitor titration]] (SUIT) protocols | |||
Classes of SUIT protocols with coupling and substrate control'''</big></big> | |||
# '''Linear coupling control''': at a single substrate state (classical State 3 -> State 4). | == Definition of states == | ||
Β | |||
=== Define substrates === | |||
* CI: PM, GM, PGM, M, .. | |||
* CII: S(Rot), S, SM, .. | |||
* CI&II: PS, GS, PMS, GMS, PGMS, .. | |||
* CIV: AsTM | |||
* FAO: Oct, Pal, ... | |||
* CGPDH: | |||
* c | |||
Β | |||
=== Define coupling states === | |||
* ''L'', ''P'', ''E'' | |||
Β | |||
=== Define residual oxygen consumption === | |||
* ROX: Rot&Ama, Rot&Mna&Ama, Cn, Az, .. | |||
Β | |||
Β | |||
== Substrate control states == | |||
Β | |||
=== Single substrate control states === | |||
Β | |||
# CI | |||
# CII | |||
# CI<small>&</small>CII | |||
# CIV | |||
# FAO | |||
# CGPDH | |||
Β | |||
=== Multiple substrate control states === | |||
Β | |||
'''A. CI-CII-CIV''' | |||
# CI + CII | |||
# CI + CII + CIV | |||
# CI + CI<small>&</small>CII + CII | |||
# CI + CI<small>&</small>CII + CII + CIV | |||
Β | |||
'''B. FAO-CI-CII-CIV''' | |||
# FAO + CI + CII | |||
# FAO + CI + CII + CIV | |||
# FAO + CI + CI<small>&</small>CII + CII | |||
# FAO + CI + CI<small>&</small>CII + CII + CIV | |||
Β | |||
'''C. CGPDH-CI-CII-CIV''' | |||
# CGPDH + CI | |||
# CGPDH + CI + CII | |||
# CGPDH + CI + CII + CIV | |||
# CGPDH + CI + CI<small>&</small>CII + CII | |||
# CGPDH + CI + CI<small>&</small>CII + CII + CIV | |||
# CI + CGPDH + ... | |||
# CI + CI<small>&</small>CII + CGPDH + ... | |||
# CI<small>&</small>CII + CGPDH + ... | |||
Β | |||
'''D. FAO-CGPDH-CI-CII-CIV''' | |||
# FAO + CGPDH + CI + CII | |||
# FAO + CGPDH + CI + CII + CIV | |||
# FAO + CGPDH + CI + CI<small>&</small>CII + CII | |||
# FAO + CGPDH + CI + CI<small>&</small>CII + CII + CIV | |||
# ... | |||
Β | |||
Β | |||
== Control patterns == | |||
=== Linear === | |||
Β | |||
# '''Linear coupling control''': at a single substrate state (classical State 3 -> State 4, or ''P'' -> ''L''<sub>T</sub>). | |||
# '''Linear substrate control''': at a single coupling state (unusual). | # '''Linear substrate control''': at a single coupling state (unusual). | ||
# '''Monodirectional linear coupling control''': ''L''<sub>N</sub> -> ''P''<sub>D</sub> -> ''E''<sub>F</sub> | # '''Monodirectional linear coupling control''': ''L''<sub>N</sub> -> ''P''<sub>D</sub> -> ''E''<sub>F</sub> | ||
# '''Bidirectional linear coupling control''': ''L''<sub>N</sub> -> ''P''<sub>D</sub> -> ''L''<sub>Omy</sub> -> ''E''<sub>F</sub> | # '''Bidirectional linear coupling control''': ''L''<sub>N</sub> -> ''P''<sub>D</sub> -> ''L''<sub>Omy</sub> -> ''E''<sub>F</sub> | ||
== Orthogonal == | === Orthogonal === | ||
# '''Orthogonal coupling -> substrate control''' (mono- or bidirectional) | # '''Orthogonal coupling -> substrate control''' (mono- or bidirectional) | ||
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== Diametral == | === Diametral === | ||
# '''Diametral coupling -> substrate ->> control''' (mono- or bidirectional) | # '''Diametral coupling -> substrate ->> control''' (mono- or bidirectional) | ||
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= References and | == References and notes == | ||
* [[MitoPedia: Respiratory states]] | * [[MitoPedia: Respiratory states]] |
Revision as of 03:18, 19 August 2015
This page is in preparation (intern wiki: SUIT protocol library intern)
Substrate-uncoupler-inhibitor titration (SUIT) protocols
Classes of SUIT protocols with coupling and substrate control
Definition of states
Define substrates
- CI: PM, GM, PGM, M, ..
- CII: S(Rot), S, SM, ..
- CI&II: PS, GS, PMS, GMS, PGMS, ..
- CIV: AsTM
- FAO: Oct, Pal, ...
- CGPDH:
- c
Define coupling states
- L, P, E
Define residual oxygen consumption
- ROX: Rot&Ama, Rot&Mna&Ama, Cn, Az, ..
Substrate control states
Single substrate control states
- CI
- CII
- CI&CII
- CIV
- FAO
- CGPDH
Multiple substrate control states
A. CI-CII-CIV
- CI + CII
- CI + CII + CIV
- CI + CI&CII + CII
- CI + CI&CII + CII + CIV
B. FAO-CI-CII-CIV
- FAO + CI + CII
- FAO + CI + CII + CIV
- FAO + CI + CI&CII + CII
- FAO + CI + CI&CII + CII + CIV
C. CGPDH-CI-CII-CIV
- CGPDH + CI
- CGPDH + CI + CII
- CGPDH + CI + CII + CIV
- CGPDH + CI + CI&CII + CII
- CGPDH + CI + CI&CII + CII + CIV
- CI + CGPDH + ...
- CI + CI&CII + CGPDH + ...
- CI&CII + CGPDH + ...
D. FAO-CGPDH-CI-CII-CIV
- FAO + CGPDH + CI + CII
- FAO + CGPDH + CI + CII + CIV
- FAO + CGPDH + CI + CI&CII + CII
- FAO + CGPDH + CI + CI&CII + CII + CIV
- ...
Control patterns
Linear
- Linear coupling control: at a single substrate state (classical State 3 -> State 4, or P -> LT).
- Linear substrate control: at a single coupling state (unusual).
- Monodirectional linear coupling control: LN -> PD -> EF
- Bidirectional linear coupling control: LN -> PD -> LOmy -> EF
Orthogonal
- Orthogonal coupling -> substrate control (mono- or bidirectional)
- Orthogonal substrate -> coupling control (mono- or bidirectional)
Diametral
- Diametral coupling -> substrate ->> control (mono- or bidirectional)
- Diametral substrate -> coupling ->> control (mono- or bidirectional)