Difference between revisions of "SUIT protocol library"
From Bioblast
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== CI - CI+CII - CII == | == CI - CI+CII - CII == | ||
=== GM+D+S+F+Rot === | |||
# GM+D+c+S+F+Rot: [[Cytochrome_c|cyt c]] added after ADP ([[Boushel 2007 Diabetologia]]) | |||
# GM+D+S+c+F+Rot: cyt c added after S | |||
* ADP conc: | |||
* ROX: Ama ([[Boushel 2007 Diabetologia]]) | |||
=== GM+D+P+S+F+Rot === | |||
# ''Innsbruck protocol 1'': GM+D+c+P+S+F+Rot: early addition of [[Cytochrome c|''c'']] ensures comparability of all states in case of any ''c'' effect | |||
# ''Innsbruck protocol 2'': PM+D+c+G+S+F+Rot | |||
* Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) | |||
* ([[Gnaiger_2007_MitoPathways]]) | |||
=== PMG+D+S+F+Rot === | === PMG+D+S+F+Rot === | ||
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* ADP conc: in mouse brain homogenate a final conc. of 2 mM ADP in the [[OXPHOS]] state (CI+CII) is saturating ([[Sumbalova_2011_Abstract]]) | * ADP conc: in mouse brain homogenate a final conc. of 2 mM ADP in the [[OXPHOS]] state (CI+CII) is saturating ([[Sumbalova_2011_Abstract]]) | ||
* [[ROX]]: Mna+Ama; Myx ([[Gnaiger_2009_IJBCB]]) | * [[ROX]]: Mna+Ama; Myx ([[Gnaiger_2009_IJBCB]]) | ||
== ETF + CI == | == ETF + CI == | ||
Revision as of 13:46, 29 September 2011
CI
CII
CI+CII
CI - CI+CII - CII
GM+D+S+F+Rot
- GM+D+c+S+F+Rot: cyt c added after ADP (Boushel 2007 Diabetologia)
- GM+D+S+c+F+Rot: cyt c added after S
- ADP conc:
- ROX: Ama (Boushel 2007 Diabetologia)
GM+D+P+S+F+Rot
- Innsbruck protocol 1: GM+D+c+P+S+F+Rot: early addition of c ensures comparability of all states in case of any c effect
- Innsbruck protocol 2: PM+D+c+G+S+F+Rot
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E)
- (Gnaiger_2007_MitoPathways)
PMG+D+S+F+Rot
- PMG+D+c+S+F+Rot
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E)
- ADP conc: in mouse brain homogenate a final conc. of 2 mM ADP in the OXPHOS state (CI+CII) is saturating (Sumbalova_2011_Abstract)
- ROX: Mna+Ama; Myx (Gnaiger_2009_IJBCB)
