Difference between revisions of "Saraiva 2022 Pathogens"
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|title=Saraiva FMS, Cosentino-Gomes D, Inacio JDF, Almeida-Amaral EE, Louzada-Neto O, Rossini A, Nogueira NP, Meyer-Fernandes JR, Paes MC (2022) Hypoxia effects on ''Trypanosoma cruzi'' epimastigotes proliferation, differentiation, and energy metabolism. https://doi.org/10.3390/pathogens11080897 | |title=Saraiva FMS, Cosentino-Gomes D, Inacio JDF, Almeida-Amaral EE, Louzada-Neto O, Rossini A, Nogueira NP, Meyer-Fernandes JR, Paes MC (2022) Hypoxia effects on ''Trypanosoma cruzi'' epimastigotes proliferation, differentiation, and energy metabolism. https://doi.org/10.3390/pathogens11080897 | ||
|info=Pathogens 11:897. [https://pubmed.ncbi.nlm.nih.gov/36015018 PMID: 36015018 Open Access] | |info=Pathogens 11:897. [https://pubmed.ncbi.nlm.nih.gov/36015018 PMID: 36015018 Open Access] | ||
|authors=Saraiva | |authors=Francis M S Saraiva 1 , Daniela Cosentino-Gomes 2 , Job D F Inacio 3 , Almeida-Amaral Elmo E, Louzada-Neto Orlando, Rossini Ana , Nogueira Natalia P, Meyer-Fernandes Jose R, Paes Marcia C | ||
|year=2022 | |year=2022 | ||
|journal=Pathogens | |journal=Pathogens | ||
Revision as of 13:19, 22 November 2022
| Saraiva FMS, Cosentino-Gomes D, Inacio JDF, Almeida-Amaral EE, Louzada-Neto O, Rossini A, Nogueira NP, Meyer-Fernandes JR, Paes MC (2022) Hypoxia effects on Trypanosoma cruzi epimastigotes proliferation, differentiation, and energy metabolism. https://doi.org/10.3390/pathogens11080897 |
» Pathogens 11:897. PMID: 36015018 Open Access
Francis M S Saraiva 1, Daniela Cosentino-Gomes 2, Job D F Inacio 3, Almeida-Amaral Elmo E, Louzada-Neto Orlando, Rossini Ana, Nogueira Natalia P, Meyer-Fernandes Jose R, Paes Marcia C (2022) Pathogens
Abstract: Trypanosoma cruzi, the causative agent of Chagas disease, faces changes in redox status and nutritional availability during its life cycle. However, the influence of oxygen fluctuation upon the biology of T. cruzi is unclear. The present work investigated the response of T. cruzi epimastigotes to hypoxia. The parasites showed an adaptation to the hypoxic condition, presenting an increase in proliferation and a reduction in metacyclogenesis. Additionally, parasites cultured in hypoxia produced more reactive oxygen species (ROS) compared to parasites cultured in normoxia. The analyses of the mitochondrial physiology demonstrated that hypoxic condition induced a decrease in both oxidative phosphorylation and mitochondrial membrane potential (ΔΨm) in epimastigotes. In spite of that, ATP levels of parasites cultivated in hypoxia increased. The hypoxic condition also increased the expression of the hexokinase and NADH fumarate reductase genes and reduced NAD(P)H, suggesting that this increase in ATP levels of hypoxia-challenged parasites was a consequence of increased glycolysis and fermentation pathways. Taken together, our results suggest that decreased oxygen levels trigger a shift in the bioenergetic metabolism of T. cruzi epimastigotes, favoring ROS production and fermentation to sustain ATP production, allowing the parasite to survive and proliferate in the insect vector. • Keywords: ROS, Trypanosoma cruzi, Bioenergetics, Hypoxia, Parasite metabolism • Bioblast editor: Plangger M
Labels: MiParea: Respiration
HRR: Oxygraph-2k
2022-11
