Sjoevall 2013 Mitochondrion: Difference between revisions
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{{Labeling | {{Labeling | ||
|area=Respiration, Instruments;methods, mt-Medicine, Patients | |area=Respiration, Instruments;methods, mt-Medicine, Patients | ||
|diseases=Aging;senescence, Neurodegenerative | |||
|injuries=Ischemia-reperfusion | |||
|organism=Human | |organism=Human | ||
|tissues=Blood cells | |tissues=Blood cells, Platelet | ||
|preparations=Intact cells, Permeabilized cells | |preparations=Intact cells, Permeabilized cells | ||
|couplingstates=LEAK, ROUTINE, OXPHOS, ETS | |couplingstates=LEAK, ROUTINE, OXPHOS, ETS | ||
|pathways=N, ROX | |pathways=N, ROX | ||
Revision as of 16:19, 9 November 2016
| Sjövall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, Arnbjörnsson E, Hansson Magnus J, Fellman V, Elmér E (2013) Mitochondrial respiration in human viable platelets - methodology and influence of gender, age and storage. Mitochondrion 13:7-14. |
Sjoevall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, Arnbjoernsson E, Hansson Magnus J, Fellman V, Elmer E (2013) Mitochondrion
Abstract: Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability. • Keywords: Diagnostics; Human; Platelets; Mitochondria; OXPHOS; High-resolution respirometry; Mitochondrial disorders
• O2k-Network Lab: SE Lund Elmer E
Labels: MiParea: Respiration, Instruments;methods, mt-Medicine, Patients
Pathology: Aging;senescence, Neurodegenerative
Stress:Ischemia-reperfusion
Organism: Human
Tissue;cell: Blood cells, Platelet
Preparation: Intact cells, Permeabilized cells
Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
Pathway: N, ROX
HRR: Oxygraph-2k
Blood cell preparation
O2k-Publications
>>Bioblast_alert_2014(02)
