Difference between revisions of "Sodium fluoride"
From Bioblast
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== References == | == References == | ||
::::* [ | ::::* [[Chinopoulos 2014 Methods Enzymol]] | ||
{{MitoPedia concepts}} | {{MitoPedia concepts}} |
Revision as of 12:58, 26 April 2024
Description
Sodium fluoride (NaF) is used in combination with beryllium sulfate to form beryllium trifluoride (BeF3โ), to inhibit the ATP synthase if it is exposed by disruption of the mitochondrial membranes.
Abbreviation: NaF
Application in HRR
- NaF: (sodium fluoride; NaF), Sigma-Aldrich: S7920, store at RT,
- Preparation of 500 mM stock solution (dissolved in H2O)
- Weigh 209.95 mg of NaF.
- Dissolve in 10 mL distilled H2O under the fume hood.
- Divide in aliquots.
- Store at +4 ยฐC.
- Preparation of 500 mM stock solution (dissolved in H2O)
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 20 ยตL using a 25 ยตL syringe (2 mL O2k-chamber).
- Final concentration: 5 mM.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
References
MitoPedia topics: Inhibitor