Souza da Silva 2020 Mol Neurobiol: Difference between revisions
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|area=Respiration | |area=Respiration, Pharmacology;toxicology | ||
|instruments=Oxygraph-2k | |diseases=Alzheimer's | ||
|additional=2020-08 | |organism=Mouse | ||
|tissues=Nervous system | |||
|preparations=Homogenate | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=N, S, NS, ROX | |||
|instruments=Oxygraph-2k, O2k-Fluorometer | |||
|additional=2020-08, AmR | |||
}} | }} |
Revision as of 18:59, 21 August 2020
da Silva JS, Nonose Y, Rohden F, Lukasewicz Ferreira PC, Fontella FU, Rocha A, Brochier AW, Apel RV, de Lima TM, Seminotti B, Amaral AU, Galina A, Souza DO (2020) Guanosine neuroprotection of presynaptic mitochondrial calcium homeostasis in a mouse study with amyloid-Ξ² oligomers. Mol Neurobiol [Epub ahead of print]. |
da Silva JS, Nonose Y, Rohden F, Lukasewicz Ferreira PC, Fontella FU, Rocha A, Brochier AW, Apel RV, de Lima TM, Seminotti B, Amaral AU, Galina A, Souza DO (2020) Mol Neurobiol
Abstract: Amyloid-Ξ² oligomers (AΞ²Os) toxicity causes mitochondrial dysfunction, leading to synaptic failure in Alzheimer's disease (AD). Considering presynaptic high energy demand and tight Ca2+ regulation, impairment of mitochondrial function can lead to deteriorated neural activity and cell death. In this study, an AD mouse model induced by ICV (intracerebroventricular) injection of AΞ²Os was used to investigate the toxicity of AΞ²Os on presynaptic function. As a therapeutic approach, GUO (guanosine) was given by oral route to evaluate the neuroprotective effects on this AD model. Following 24 h and 48 h from the model induction, behavioral tasks and biochemical analyses were performed, respectively. AΞ²Os impaired object recognition (OR) short-term memory and reduced glutamate uptake and oxidation in the hippocampus. Moreover, AΞ²Os decreased spare respiratory capacity, reduced ATP levels, impaired Ca2+ handling, and caused mitochondrial swelling in hippocampal synaptosomes. Guanosine crossed the BBB, recovered OR short-term memory, reestablished glutamate uptake, recovered mitochondrial Ca2+ homeostasis, and partially prevented mitochondrial swelling. Therefore, this endogenous purine presented a neuroprotective effect on presynaptic mitochondria and should be considered for further studies in AD models. β’ Keywords: Alzheimerβs disease, Amyloid-Ξ² oligomers, Guanosine, Neuroprotection, Presynaptic mitochondria β’ Bioblast editor: Plangger M
Labels: MiParea: Respiration, Pharmacology;toxicology
Pathology: Alzheimer's
Organism: Mouse Tissue;cell: Nervous system Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k, O2k-Fluorometer
2020-08, AmR