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• Kuznetsov 1999 Transplant Proc
  ...AV, Brandacher G, Steurer W, Margreiter R, Gnaiger E (1999) Estimation of mitochondrial damage in heart preservation. Transplant Proc 31:992. ...spirometry with permeabilized cells and muscle fibers for investigation of mitochondrial cold ischemia/reperfusion (CIR) injury.
  1 KB (120 words) - 04:24, 23 November 2021
• Peyta 2016 Biochim Biophys Acta
  ...content decreases respiratory chain capacities and increases ATP synthesis yield in the human HepaRG cells. Biochim Biophys Acta 1857:443-53. ...entation. In addition, CL content may regulate mitochondrial ATP synthesis yield.
  3 KB (392 words) - 23:54, 18 March 2018
• Cardoso 2022 MitoFit rTCA
  |title=Cardoso LHD, Gnaiger E (2022) Reverse citric acid cycle and mitochondrial succinate formation under anoxia: from parasites and invertebrates to ische ...t profound implications on the redox balance (Harrison et al 2015) and ATP yield (Gnaiger 1991).
  2 KB (349 words) - 10:00, 26 February 2022
• Stankova 2017 MiP2017
  ...paration technique depends on the requirements regarding organelle purity, yield, activity and also the duration of the preparation. The most widely used me ...production (DCFDA) and mitochondrial membrane potential (Safranine O). The yield, purity and the loss of mitochondria were monitored by measuring enzyme act
  4 KB (548 words) - 10:56, 18 March 2020
• Xia 2016 RSC Adv
  ...lly, compared with the PCV2 yield of infected control PK15 cells, the PCV2 yield of infected Aralar I over-expression cells was enhanced by 42.5% (p < 0.05)
  2 KB (279 words) - 13:21, 25 February 2020
• Mueller 2023 Int J Mol Sci
  ...Donhauser E, Maske T, Bischof C, Dumitrescu D, Rudolph V, Klinke A (2023) Mitochondrial integrity is critical in right heart failure development. Int J Mol Sci 24: ...clinical and clinical RVD and RHF and to decipher the relationship between mitochondrial processes and the functional aspects of the right ventricle (RV).
  2 KB (220 words) - 17:48, 13 October 2023
• Long 2022 Cell Commun Signal
  ...sing RT-qPCR, the sarcomere organization using immunofluorescence, and the mitochondrial function using oxidative respiration analysis. ...g contractile- and ion transport-associated gene expression, and enhancing mitochondrial respiration. M0 macrophages did not significantly affect the cardiomyogenes
  3 KB (390 words) - 14:49, 31 August 2022
• Goo 2013 Clin Exp Pharmacol Physiol
  ...10%-15%. Mitochondrial experiments invoke a trio of titration protocols to yield information on oxygen consumption, ATP flux, membrane potential, electron l ...t production, Mitochondrial membrane potential, Mitochondrial proton leak, Mitochondrial reactive oxygen species production
  2 KB (222 words) - 11:40, 9 February 2022
• Lai 2019 Acta Physiol (Oxf)
  ...Kummitha CM, Rosca MG, Fujioka H, Tandler B, Hoppel CL (2019) Isolation of mitochondrial subpopulations from skeletal muscle: Optimizing recovery and preserving int ...ehensive approach is developed to identify the best conditions to separate mitochondrial fractions.
  2 KB (317 words) - 08:44, 16 July 2021
• Exergy
  Changes of exergy d''G'' plus [[bound energy]] yield the [[enthalpy]] change: :::# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th e
  1 KB (134 words) - 05:57, 16 September 2022
• Werner 2010 Meat Sci
  |title=Werner C, Natter R, Schellander K, Wicke M (2010) Mitochondrial respiratory activity in porcine longissimus muscle fibers of different pig ...larger than those of the other genetics (''P''<0.05). The analysis of the mitochondrial respiratory activity (MRA) using permeabilized longissimus muscle fibers re
  2 KB (244 words) - 10:51, 27 March 2018
• Divakaruni 2014 Curr Protoc Toxicol
  |title=Divakaruni AS, Rogers GW, Murphy AN (2014) Measuring mitochondrial function in permeabilized cells using the Seahorse XF Analyzer or a clark-t ...tic understanding. Mitochondria can be isolated from intact cells, but the yield and quality of the end product is often poor and prone to subselection duri
  2 KB (229 words) - 17:46, 9 November 2017
• Lai 2018 Acta Physiol (Oxf)
  ...Kummitha C, Rosca MG, Fujioka H, Tandler B, Hoppel CL (2018) Isolation of mitochondrial subpopulations from skeletal muscle: optimizing recovery and preserving int ...ehensive approach is developed to identify the best conditions to separate mitochondrial fractions.
  3 KB (362 words) - 17:49, 25 January 2021
• Trifunovic 2019 MiP2019
  ...the largest enzyme of respiratory chain and the most commonly affected in mitochondrial diseases. Mammalian CI consists of 44 different subunits organized in a mod ...n dysfunction and identifies CLPP as an exciting new target for therapy of mitochondrial diseases.
  2 KB (315 words) - 14:55, 8 October 2019
• Rasmussen 1997 Mol Cell Biochem
  ...relative to mitochondrial protein. The pig tissue contains ten times less mitochondrial protein than the pigeon tissue does.
  2 KB (309 words) - 01:21, 16 February 2022
• Taroni 1987 Biochim Biophys Acta
  |title=Taroni F, Gellera C, Di Donato S (1987) Evidence for two distinct mitochondrial malic enzymes in human skeletal muscle: Purification and properties of the ...raphy. The NAD+/NADP+-dependent enzyme was purified 1600-fold with a final yield of 34% and a final specific activity of 32.9 units/mg of protein by employi
  2 KB (272 words) - 15:10, 27 May 2020
• Steurer 1999 Transplant Proc
  ...ed, islet isolation still suffers from large variability in terms of islet yield and quality. A comprehensive quality control for isolated and purified isle ...clinical setting. We hypothesize, that analysis of membrane integrity and mitochondrial function by high-resolution respirometry may provide a guideline for furthe
  2 KB (282 words) - 04:24, 23 November 2021
• Sperl 1994 J Inher Metab Dis
  ...4) Polarographic studies of saponin-skinned muscle fibers in patients with mitochondrial myopathies. J Inher Metab Dis 17:307-10. ...''., 1987) and human muscle tissue. In such permeabilized fibers, study of mitochondrial respiratory control is possible as in isolated mitochondria but without the
  2 KB (331 words) - 04:39, 23 November 2021
• Dawson 2022 FASEB J
  ...in high-altitude deer mice. Therefore, physiological variation in specific mitochondrial fractions can help overcome the metabolic challenges of life at high altitu
  3 KB (340 words) - 17:44, 23 November 2022
• Ikaga 2015 Biochem Biophys Rep
  ...hend MR, Tanakac H, Iida-Tanakab N (2015) Knockdown of aquaporin-8 induces mitochondrial dysfunction in 3T3-L1 cells. Biochem Biophys Rep 4:187–95. ...nctions on the plasma membrane and/or on the mitochondrial inner membrane. Mitochondrial AQP8 with permeability for water, H₂O₂ and NH<sub>3</
  3 KB (390 words) - 14:42, 13 November 2017
• Dasika 2015 Biophys J
  ...Vinnakota KC, Beard DA (2015) Determination of the catalytic mechanism for mitochondrial malate dehydrogenase. Biophys J 108:408-19. ...ng the reaction. Previous kinetic studies on the mitochondrial MDH did not yield a consensus kinetic model that explains both substrate and pH dependency of
  2 KB (258 words) - 08:46, 17 December 2018
• O2k-mitochondrial preparations
  == O2k-Mitochondrial preparations == ...dria with well preserved function is a prerequisite for the measurement of mitochondrial respiration. In the literature a wide diversity of buffers with different c
  2 KB (334 words) - 11:21, 28 March 2024
• Griffiths 1995 Biochem J
  |title=Griffiths EJ, Halestrap AP (1995) Mitochondrial non-specific pores remain closed during cardiac ischaemia, but open upon re ...ay occur during the initial phase of reperfusion. The apparent increase in mitochondrial [3H]DOG in the CsA-perfused hearts is explained by the ability of the drug
  3 KB (369 words) - 08:36, 16 June 2015
• Meza-Buendia 2022 PLoS One
  ...ately 3 to 4 hours from isolation of intact mitochondria to measurement of mitochondrial oxygen consumption.
  2 KB (312 words) - 13:36, 5 September 2022
• Gnaiger 1998 BTK-COX
  ...ty of cytochrome ''c'' oxidase in terms of “synkinetic” regulation of high mitochondrial oxygen affinity. |discipline=Mitochondrial Physiology
  3 KB (350 words) - 04:26, 23 November 2021
• Dias 2018 Anal Biochem
  ...onses were observed. This methodology can be used to detect differences in mitochondrial function at the oxidative phosphorylation level and for studies with differ
  3 KB (366 words) - 06:14, 20 April 2020
• SUIT-011
  |info='''A: Maximum mitochondrial respiratory capacity ([[Oxidative phosphorylation|OXPHOS]] with [[NS-pathwa ...) with NADH linked-substrates ([[GM-pathway control state|GM]]). GM and PM yield practically identical fluxes in human skeletal muscle fibres. However, PM i
  6 KB (951 words) - 15:35, 8 June 2020
• Cardol 2003 Plant Physiol
  ...cle C, Matagne R, Franck F (2003) Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinhardtii affected in respiration. Plant Physiol ...s demonstrate that in ''C. reinhardtii'' mutants, permanent defects in the mitochondrial electron transport chain stabilize state 2, which favors cyclic over linear
  2 KB (307 words) - 15:12, 8 August 2023
• Lashbrook 2016 Thesis
  ...’ models might yield novel insights on the effect of short-term obesity on mitochondrial function. The aim of this study is to compare mitochondrial function in a hibernator, the golden-mantled ground squirrel (''Callospermo
  3 KB (378 words) - 09:12, 8 November 2016
• Flux control efficiency
  = Flux control efficiency: normalization of mitochondrial respiration = |title=Gnaiger E (2020) Flux control efficiency: normalization of mitochondrial respiration. Mitochondr Physiol Network 2016-03-20; updated 2020-11-10.
  8 KB (1,272 words) - 18:27, 1 January 2021
• Lai 2014 Abstract1 MiP2014
  ...hich RCR were higher than those obtained in rat studies; the mitochondrial yield was slightly increased only for the IFM population in comparison to those o ...athway components [5]. Electron microscopy images of the subpopulations of mitochondrial confirmed that the procedure preserved the structure SSM and IFM.
  5 KB (680 words) - 15:18, 28 March 2018
• Dahdah 2019 MiP2019
  |title=[[File:ProfileP.jpg|left|90px|Norma Dahdah]] Approaches to assess mitochondrial respiration in white adipose tissue. ...tage of using isolated mitochondria is the high amount of tissue needed to yield one sample and the loss of damaged mitochondria through the isolation proce
  3 KB (451 words) - 14:39, 3 October 2019
• Lai 2022 Abstract Bioblast
  ...mitha CM, Hoppel CL (2022) Effect of isolation protocol of skeletal muscle mitochondrial subpopulations on bioenergetic function. '''Bioblast 2022: BEC Inaugural Co ...the bioenergetic characteristics of SSmt and IFmt with those of the whole mitochondrial population. This comparison was obtained for both control (C) and T1D rats.
  6 KB (853 words) - 08:39, 28 July 2022
• Affourtit 1998 J Biol Chem
  ...x 10(7) cells ml-1). Kinetic studies suggest that the effect on growth is mitochondrial in origin. In isolated mitochondria we found that the alternative oxidase a
  2 KB (249 words) - 16:49, 18 March 2021
• Respiratory state
  ...ined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the [[coupling-control ::: Coupling control states and ''[[CCR]]'' of mitochondrial preparations:
  3 KB (400 words) - 15:50, 15 August 2021
• Brown 2000 Biochem Educ
  |title=Brown S (2000) Does succinate oxidation yield FADH₂ or ubiquinol? Biochem Educ 28:52-4. ...the oxidation of succinate and the reduction of FAD, but in presenting the mitochondrial electron transfer chain most authors show the oxidation of succinate couple
  2 KB (299 words) - 16:26, 27 August 2023
• Affourtit 1999 J Biol Chem
  ...lls ml^-1). Kinetic studies suggest that the effect on growth is mitochondrial in origin. In isolated mitochondria we found that the alternative oxidase a
  2 KB (265 words) - 12:39, 3 April 2021
• Munro 2013 Aging Cell
  ...H₂O₂ than those of the two short-lived species in nearly all conditions of mitochondrial respiration tested, including forward, reverse, and convergent electron flo
  3 KB (376 words) - 16:44, 21 November 2018
• Ren 2014 Sci Rep
  ...malic enzyme 2 (ME2) catalyzes the oxidative decarboxylation of malate to yield CO2 and pyruvate, with concomitant reduction of dinucleotide cofactor NAD(+
  2 KB (240 words) - 14:22, 9 November 2016
• Bodis 2019 J Clin Endocrinol Metab
  ...egler D, Schuermann A, Roden M, Szendroedi J (2019) Expansion and impaired mitochondrial efficiency of deep subcutaneous adipose tissue in recent-onset type 2 diabe ...cent-onset type 2 diabetes (T2D). Understanding AT energy metabolism could yield novel targets for the treatment of T2D.
  3 KB (414 words) - 15:37, 7 May 2020
• Hughes 2015 Front Physiol
  ...L, Tahmasebi H, Laham R, Gurd BJ, Quadrilatero J, Kane DA, Perry CG (2015) Mitochondrial bioenergetics and fiber type assessments in microbiopsy vs. Bergstrom percu ...tion. Microbiopsies of human skeletal muscle can be utilized for assessing mitochondrial respiratory kinetics in PmFB when assay conditions are supplemented with BL
  3 KB (390 words) - 12:13, 4 February 2019
• Hoppel 2016b Abstract MitoFit Science Camp 2016
  ...d mitochondrial function or oxidative phosphorylation (OXPHOS) to evaluate mitochondrial dysfunction in energy production as a diagnostic tool in skeletal muscle. ...areful and quantitative evaluation of methods available for measurement of mitochondrial function is important for combining such results in a comparative data base
  3 KB (452 words) - 18:44, 10 January 2022
• Templage:Keywords-MitoPedia BEC 2020.2
  | ATP yield per O₂ || [[ATP yield]] || ''Y''_P»/O₂ || 1 || P»/O₂ ratio meas | mitochondria or mitochondrial || [[Mitochondria]] || mt || - || Box 1
  15 KB (2,142 words) - 23:29, 6 February 2021
• Rodriguez 2019 Abstract IOC141
  ...ndria as “flux capacitors”: the effect of mitonuclear incompatibilities on mitochondrial physiology, metabolomics profile and gene expression in ''D. melanogaster'' ...female flies at three time points during their lifetimes. Following these mitochondrial physiology measurements, we will look at the effects of the treatments on m
  4 KB (513 words) - 10:26, 2 September 2019
• Lemieux 2006 Biochim Biophys Acta
  ...f J-C, Gnaiger E (2006) Temperature effects on the control and capacity of mitochondrial respiration in permeabilized fibers of the mouse heart. Biochim Biophys Act ...her studies of mouse heart metabolism, including various genetic models of mitochondrial diseases and dysfunction.
  3 KB (453 words) - 18:14, 10 January 2022
• SUIT-011 O2 pfi D024
  ...NS-OXPHOS capacity provides a physiologically relevant estimate of maximum mitochondrial respiratory capacity. :::- GM and PM yield typically identical fluxes in human skeletal muscle fibres. However, PM is
  5 KB (784 words) - 11:18, 17 September 2020
• Ziviani 2016 Abstract Mito Xmas Meeting Innsbruck
  ...rkin. ''In vivo'', genetic or pharmacological inhibition of USP8 corrected mitochondrial dysfunction and locomotor performance in a ''Drosophila'' PINK1 mutant mode
  2 KB (301 words) - 17:33, 14 December 2016
• Aryaman 2015 Abstract MiPschool London 2015
  |title=Modelling mitochondrial DNA variability in metabolism. |abstract=Mutations in mitochondrial DNA are often observed in cancer, although their role is still somewhat unc
  3 KB (472 words) - 13:27, 7 November 2016
• Garedew 2006 Biochim Biophys Acta
  ...ion in the mouse heart, including genetic models of acquired and inherited mitochondrial defects. |discipline=Mitochondrial Physiology
  3 KB (510 words) - 18:14, 10 January 2022
• Ravasz 2018 Biochim Biophys Acta
  ...by complex I is inhibited by rotenone or anoxia, mitochondrial diaphorases yield NAD⁺, provided that suitable quinones are present (Kiss G et al.
  2 KB (338 words) - 13:19, 2 August 2023

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