Difference between revisions of "State 3"
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=''P'' | |abbr=''P'' | ||
|description=Whereas [[OXPHOS capacity]] is measured at saturating concentrations of ADP and Pi, '''State 3''' is defined as ADP stimulated respiration in the presence of high ADP and Pi concentrations [[ | |description=Whereas [[OXPHOS capacity]] is measured at saturating concentrations of ADP and Pi, '''State 3''' is defined as ADP stimulated respiration in the presence of high ADP and Pi concentrations [[Chance_1955_JBC-III|(Chance and Williams, 1955]]) which are not necessarily saturating.Β For instance, non-saturating ADP concentrations are applied in State 3 in pulse titrations to determine the [[P/O ratio]] in State 3β4 (DβT) transitions, when saturating ADP concentrations would deplete the oxygen concentration in the closed oxygraph chamber before [[State 4]] is obtained ([[Gnaiger_2000_PNAS|Gnaiger et al 2000]]; [[Puchowicz_2004_Mitochondrion|(Puchowicz et al 2004]]). | ||
|info=MiPNet12.15, Chance_1955_JBC-III | |info=MiPNet12.15, Chance_1955_JBC-III | ||
|type=Respiration | |type=Respiration |
Revision as of 08:47, 16 September 2010
Description
Whereas OXPHOS capacity is measured at saturating concentrations of ADP and Pi, State 3 is defined as ADP stimulated respiration in the presence of high ADP and Pi concentrations (Chance and Williams, 1955) which are not necessarily saturating. For instance, non-saturating ADP concentrations are applied in State 3 in pulse titrations to determine the P/O ratio in State 3β4 (DβT) transitions, when saturating ADP concentrations would deplete the oxygen concentration in the closed oxygraph chamber before State 4 is obtained (Gnaiger et al 2000; (Puchowicz et al 2004).
Abbreviation: P
Reference: MiPNet12.15, Chance_1955_JBC-III
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.