Stray light: Difference between revisions
From Bioblast
Harrison DK (talk | contribs) (Created page with "{{MitoPedia |abbr=n.a. |description='''Stray light''' is defined as the detected light of any wavelength that lies outside the bandwidth of the selected wavelength. In the pr...") |
Harrison DK (talk | contribs) No edit summary |
||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=n.a. | |abbr=n.a. | ||
|description='''Stray light''' is defined as the detected light of any wavelength that lies outside the [[bandwidth]] of the selected wavelength. In the presence of '''stray light''' of intensity ''I''<sub>''s''</sub>, the equation for [[transmittance]] (''T'') becomes ''T'' = (''I'' + ''I''<sub>''s''</sub>)/(''I''<sub>''0''</sub> + ''I''<sub>''s''</sub>) where ''I''<sub>''0''</sub> is the incident light intensity and ''I'' is the transmitted light intensity. Clearly, the lower the value of ''I'', the more dominant becomes the '''stray light''' term and so can cause errors in the quantification of low [[fluorescence]] signals or at high levels of [[absorbance]]. | |description='''Stray light''' is defined as the detected light of any wavelength that lies outside the [[bandwidth]] of the selected wavelength. In the presence of '''stray light''' of intensity ''I''<sub>''s''</sub>, the equation for [[transmittance]] (''T'') becomes ''T'' = (''I'' + ''I''<sub>''s''</sub>)/(''I''<sub>''0''</sub> + ''I''<sub>''s''</sub>) where ''I''<sub>''0''</sub> is the incident light intensity and ''I'' is the transmitted light intensity. Clearly, the lower the value of ''I'', the more dominant becomes the '''stray light''' term and so can cause errors in the quantification of low [[fluorescence]] signals or at high levels of [[absorbance]]. | ||
|info=Owen T (1996) Fundamentals of modern UV-visible spectroscopy. A Primer. Hewlett Packard, 142pp. | |||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods |
Revision as of 20:06, 25 November 2011
Description
Stray light is defined as the detected light of any wavelength that lies outside the bandwidth of the selected wavelength. In the presence of stray light of intensity Is, the equation for transmittance (T) becomes T = (I + Is)/(I0 + Is) where I0 is the incident light intensity and I is the transmitted light intensity. Clearly, the lower the value of I, the more dominant becomes the stray light term and so can cause errors in the quantification of low fluorescence signals or at high levels of absorbance.
Abbreviation: n.a.
Reference: Owen T (1996) Fundamentals of modern UV-visible spectroscopy. A Primer. Hewlett Packard, 142pp.
MitoPedia methods:
Fluorometry,
Spectrophotometry